Mitogenic effect of basic fibroblast growth factor and estradiol on cultured human myometrial and leiomyoma cells

Phillip N. Rauk, Urvashi Surti, James M. Roberts, George Michalopoulos

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Objective: We compared the mitogenic effect of basic fibroblast growth factor with and without estradiol on myometrial and leiomyometrial cells. Study Design: The mitogenic effect of basic fibroblast growth factor on myometrial cells was measured by thymidine incorporation and cell count. The mitogenic effect of basic fibroblast growth factor with and without estradiol as measured by thymidine incorporation was compared between myometrial and leiomyometrial cells. Results: Both human myometrial and leiomyometrial cells showed significant (p = 0.004 and p = 0.001, respectively), dose-dependent incorporation of thymidine in response to basic fibroblast growth factor. Leiomyometrial cells showed significantly (p = 0.04) less thymidine incorporation compared with matched normal myometrial cells. The addition of estradiol with basic fibroblast growth factor did not result in a further increase in thymidine incorporation. Conclusions: Both myometrial and leiomyometrial cells respond to basic fibroblast growth factor with increased thymidine incorporation; however leiomyometrial cells are less responsive than are matched normal myometrial cells. The addition of estradiol is not synergistic with basic fibroblast growth factor.

Original languageEnglish (US)
Pages (from-to)571-577
Number of pages7
JournalAmerican journal of obstetrics and gynecology
Volume173
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Bibliographical note

Funding Information:
From the Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology, and Reproductive Sciences,” and the Departments of Human Genetics” and Pathology,“. University of Pittsburgh. Supported by the Magee-Womens Hospital Foundation Research Fund. Presented at the Fortieth Annual Meeting of the Society for Gynecologic Investigation, Toronto, Ontario, Canada, March 31-April 3, 1993. Received for publication October 11, 1994; revised November 7, 1994; accepted December 15, 1994. Reprint requests: Phillip N. Rauk, MD, Department of Obstetrics, Gynecology, and Reproductive Sciences, Maeee-Womens Hospital, 360 Haget St., Pit&burgh, PA 15213. v Copyright 0 1995 by Mosby-Year Book, Inc. 0002-9378/95 $3.00 + 0 6/l/62763

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Basic fibroblast growth factor
  • estradiol
  • leiomyoma

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