Mitogen-stimulated lymphocytes release biologically active corticotropin

Benjamin L. Clarke, Bryan M. Gebhardt, J. Edwin Blalock

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We demonstrate that mitogen-activated lymphocytes release a biologically active form of ACTH that stimulates the in vitrorelease of corticosterone from cocultured rat adrenal cells. Neither nonstimulated lymphocytes nor the addition of mitogens alone to adrenal cell cultures had an effect. The steroidogenic activity could be neutralized by rabbit anti-ACTH serum, but not by a nonimmune serum. Both Concana-valin-A- and lipopolysaccharide-stimulated lymphocytes secrete an ACTH-like molecule with an antigenic specificity identical to pituitary-derived ACTH. Further, the amount of measurable immunoreactive ACTH was far lower than the amount of exogenously added ACTH required to evoke such a vigorous glucocorticoid response, suggesting that local deposition of the hormone results in a higher effective ACTH concentration. In addition, lymphocytes physically isolated from adrenal cells by a semipermeable membrane could stimulate steroidogenesis by 48 h, which corresponds to the rise in ACTH detected by RIA. These results confirm that activated lymphocytes synthesize as well as release biologically active ACTH, thus providing an in vitro model for a bidirectional communication between the endocrine and immune systems.

Original languageEnglish (US)
Pages (from-to)983-988
Number of pages6
JournalEndocrinology
Volume132
Issue number3
DOIs
StatePublished - Jan 1 1993

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Mitogens
Adrenocorticotropic Hormone
Lymphocytes
Endocrine System
Corticosterone
Serum
Glucocorticoids
Lipopolysaccharides
Epitopes
Immune System
Cell Culture Techniques
Communication
Hormones
Rabbits
Membranes

Cite this

Mitogen-stimulated lymphocytes release biologically active corticotropin. / Clarke, Benjamin L.; Gebhardt, Bryan M.; Blalock, J. Edwin.

In: Endocrinology, Vol. 132, No. 3, 01.01.1993, p. 983-988.

Research output: Contribution to journalArticle

Clarke, Benjamin L. ; Gebhardt, Bryan M. ; Blalock, J. Edwin. / Mitogen-stimulated lymphocytes release biologically active corticotropin. In: Endocrinology. 1993 ; Vol. 132, No. 3. pp. 983-988.
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