Mitochondrial genome sequencing reveals potential origins of the scabies mite Sarcoptes scabiei infesting two iconic Australian marsupials

Tamieka A. Fraser, Renfu Shao, Nicholas M. Fountain-Jones, Michael Charleston, Alynn Martin, Pam Whiteley, Roz Holme, Scott Carver, Adam Polkinghorne

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Background: Debilitating skin infestations caused by the mite, Sarcoptes scabiei, have a profound impact on human and animal health globally. In Australia, this impact is evident across different segments of Australian society, with a growing recognition that it can contribute to rapid declines of native Australian marsupials. Cross-host transmission has been suggested to play a significant role in the epidemiology and origin of mite infestations in different species but a chronic lack of genetic resources has made further inferences difficult. To investigate the origins and molecular epidemiology of S. scabiei in Australian wildlife, we sequenced the mitochondrial genomes of S. scabiei from diseased wombats (Vombatus ursinus) and koalas (Phascolarctos cinereus) spanning New South Wales, Victoria and Tasmania, and compared them with the recently sequenced mitochondrial genome sequences of S. scabiei from humans. Results: We found unique S. scabiei haplotypes among individual wombat and koala hosts with high sequence similarity (99.1% - 100%). Phylogenetic analysis of near full-length mitochondrial genomes revealed three clades of S. scabiei (one human and two marsupial), with no apparent geographic or host species pattern, suggestive of multiple introductions. The availability of additional mitochondrial gene sequences also enabled a re-evaluation of a range of putative molecular markers of S. scabiei, revealing that cox1 is the most informative gene for molecular epidemiological investigations. Utilising this gene target, we provide additional evidence to support cross-host transmission between different animal hosts. Conclusions: Our results suggest a history of parasite invasion through colonisation of Australia from hosts across the globe and the potential for cross-host transmission being a common feature of the epidemiology of this neglected pathogen. If this is the case, comparable patterns may exist elsewhere in the 'New World'. This work provides a basis for expanded molecular studies into mange epidemiology in humans and animals in Australia and other geographic regions.

Original languageEnglish (US)
Article number233
JournalBMC evolutionary biology
Volume17
Issue number1
DOIs
StatePublished - Nov 28 2017

Bibliographical note

Funding Information:
This work was funded by the Holsworth Wildlife Research Endowment to TF, SC and AP, and the Cradle Coast NRM to SC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Keywords

  • Conservation
  • Koala
  • Mitochondrial genome sequencing
  • Phylogeny
  • Sarcoptes scabiei
  • Wombat
  • cox1

Fingerprint Dive into the research topics of 'Mitochondrial genome sequencing reveals potential origins of the scabies mite Sarcoptes scabiei infesting two iconic Australian marsupials'. Together they form a unique fingerprint.

Cite this