MiR-886-3p down regulates CXCL12 (SDF1) expression in human marrow stromal cells

Manoj M. Pillai, Xiaodong Yang, Ilango Balakrishnan, Lynne Bemis, Beverly Torok-Storb

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Stromal Derived Factor 1 (SDF1 or CXCL12), is a chemokine known to be critical for the migration of cells in several tissue systems including the homing of the hematopoietic stem cell (HSC) to its niche in the bone marrow. A comparative analysis of miRNA expression profiles of two stromal cell lines, distinguishable by function and by CXCL12 expression (CXCL12+ and CXCL12-), revealed that the CXCL12- cells expressed >40 fold more miR-886-3p than the CXCL12+ cells. Screening studies showed that when miR-886-3p was transfected into the CXCL12+ stromal cells, the expression of CXCL12 was downregulated by as much as 85% when compared to appropriate controls, and results in the loss of CXCL12-directed chemotaxis. Similar reductions in CXCL12 were obtained with the transfection of miR-886-3p into primary stromal cell cultures. Additional studies showed that miR-886-3p specifically targeted the 3′ untranslated region (UTR) of CXCL12 mRNA. These data suggest a role for miRNA in modulating the expression of CXCL12, a gene product with a critical role in hematopoietic regulation.

Original languageEnglish (US)
Article numbere14304
JournalPloS one
Volume5
Issue number12
DOIs
StatePublished - Dec 1 2010

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stromal cells
Stromal Cells
MicroRNAs
Down-Regulation
Bone Marrow
Cells
microRNA
3' Untranslated Regions
Stem cells
Chemokines
Cell culture
Screening
Bone
Primary Cell Culture
Genes
chemotaxis
3' untranslated regions
Chemotaxis
chemokines
transfection

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MiR-886-3p down regulates CXCL12 (SDF1) expression in human marrow stromal cells. / Pillai, Manoj M.; Yang, Xiaodong; Balakrishnan, Ilango; Bemis, Lynne; Torok-Storb, Beverly.

In: PloS one, Vol. 5, No. 12, e14304, 01.12.2010.

Research output: Contribution to journalArticle

Pillai, Manoj M. ; Yang, Xiaodong ; Balakrishnan, Ilango ; Bemis, Lynne ; Torok-Storb, Beverly. / MiR-886-3p down regulates CXCL12 (SDF1) expression in human marrow stromal cells. In: PloS one. 2010 ; Vol. 5, No. 12.
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