Minimum inhibitory concentration of Brazilian Brachyspira hyodysenteriae strains

Amanda G.S. Daniel, José P.H. Sato, Michelle P. Gabardo, Talita P. Resende, David E.S.N. de Barcellos, Carlos E.R. Pereira, Fábio A. Vannucci, Roberto M.C. Guedes

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The objectives of this study were to characterize Brachyspira hyodysenteriae isolates and to evaluate the antimicrobial susceptibility patterns of strains obtained from pigs in Brazil based on the minimal inhibitory concentration test (MIC). The MIC was performed for 22 B. hyodysenteriae isolates obtained from 2011 to 2013 using the following antimicrobial drugs: tylosin, tiamulin, valnemulin, doxycycline, lincomycin and tylvalosin. Outbreaks of swine dysentery were diagnosed based on clinical presentation, bacterial isolation, gross and microscopic lesions, duplex PCR for B. hyodysenteriae and B. pilosicoli and nox gene sequencing. All obtained MIC values were consistently higher or equal to the microbiological cut-offdescribed in the literature. The MIC 90 values for the tested drugs were 8μg/ml for doxycycline, > 4μg/ml for valnemulin, 8μg/ml for tiamulin, 32μg/ml for tylvalosin, > 64μg/ml for lincomycin and > 128μg/ml for tylosin. These results largely corroborate those reported in the literature. Tiamulin, doxycycline and tylvalosin showed the lowest MIC results. All of the samples subjected to phylogenetic analysis based on the nox gene sequence exhibited similar results, showing 100% identity to B. hyodysenteriae. This is the first study describing the MIC pattern of B. hyodysenteriae isolated in Brazil.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalPesquisa Veterinaria Brasileira
Issue number4
StatePublished - 2017

Bibliographical note

Funding Information:
We thank Connie Gebhart at the University of Minnesota Veterinary Diagnostic Laboratory and Dr. Jill Thomson of Scottish Agricultural College for their technical support. We thank Capes, Fapemig and CNPq for financial support. RMC Guedes has a research fellowship from CNPq.


  • Antimicrobial sensitivity
  • Brachyspira hyodysenteriae
  • Diarrhea
  • Inhibitory concentration
  • MIC
  • Nox gene
  • Swine dysentery


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