Minimization of a sodium dithionite-derived interference in nitrate reductase-methyl viologen reactions

David R. Senn, Peter W. Carr, Leon N. Klatt

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The use of sodium dithionite as the reducing agent to produce the radical cation of methyl viologen cofactor for the nitrate reductase-catalyzed reduction of nitrate to nitrite causes a negative interference in the diazonium salt azo dye determination of nitrite. The interference results from bisulfite, a reaction product of dithionite, reacting with the diazonium salt formed from nitrite and sulfanilamide. Addition of formaldehyde before the diazotization reaction masks this interference but at low acidity introduces a nonlinearity in the nitrite calibration curve. This nonlinearity is due to a competing reaction of formaldehyde with sulfanilamide to yield a Schiff base and can be eliminated by increasing the HCL content of the sulfanilamide reagent.

Original languageEnglish (US)
Pages (from-to)464-471
Number of pages8
JournalAnalytical Biochemistry
Volume75
Issue number2
DOIs
StatePublished - Oct 1976

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