Miniaturized device for whole cortex mesoscale imaging in freely behaving mice

Mathew L. Rynes, Daniel Surinach, Micheal Laroque, Samantha Linn, Judith Dominguez, Leila Ghanbari, Suhasa B. Kodandaramaiah

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The advent of genetically-encoded calcium indicators, along with surgical preparations such as thinned skulls or refractive index matched skulls, have enabled mesoscale cortical activity imaging in headfixed mice. Such imaging studies have revealed complex patterns of coordinated activity acroß the cortical surface during a variety of behaviors. However, neural activity during free, unrestrained behavior significantly differs from that observed in headfixed animals. The ability to perform mesoscale imaging of the cortex in freely behaving mice may launch new avenues of scientific enquiry. Here we present the 'Mesoscope', a miniature, head-mountable imaging device compatible with transparent polymer skulls recently developed by our group. With an 8x10 mm field of view, the Mesoscope can image most of the mouse dorsal cortex and weighs only 3.7 grams. Here, we show preliminary behavioral experiments and mesoscale imaging in anesthetized mice.

Original languageEnglish (US)
Title of host publicationNeural Imaging and Sensing 2020
EditorsQingming Luo, Jun Ding, Ling Fu
ISBN (Electronic)9781510632158
StatePublished - 2020
EventNeural Imaging and Sensing 2020 - San Francisco, United States
Duration: Feb 3 2020Feb 5 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceNeural Imaging and Sensing 2020
Country/TerritoryUnited States
CitySan Francisco

Bibliographical note

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