Mimicking Class I b Mn2-Ribonucleotide Reductase: A MnII2 Complex and Its Reaction with Superoxide

Adriana M. Magherusan, Ang Zhou, Erik R. Farquhar, Max García-Melchor, Brendan Twamley, Lawrence Que, Aidan R. McDonald

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A fascinating discovery in the chemistry of ribonucleotide reductases (RNRs) has been the identification of a dimanganese (Mn2) active site in class I b RNRs that requires superoxide anion (O2.−), rather than dioxygen (O2), to access a high-valent Mn2 oxidant. Complex 1 ([Mn2(O2CCH3)(N-Et-HPTB)](ClO4)2, N-Et-HPTB=N,N,N′,N′-tetrakis(2-(1-ethylbenzimidazolyl))-2-hydroxy-1,3-diaminopropane) was synthesised in high yield (90 %). 1 was reacted with O2.− at −40 °C resulting in the formation of a metastable species (2). 2 displayed electronic absorption features (λmax=460, 610 nm) typical of a Mn-peroxide species and a 29-line EPR signal typical of a MnIIMnIII entity. Mn K-edge X-ray absorption near-edge spectroscopy (XANES) suggested a formal oxidation state change of MnII2 in 1 to MnIIMnIII for 2. Electrospray ionisation mass spectrometry (ESI-MS) suggested 2 to be a MnIIMnIII-peroxide complex. 2 was capable of oxidizing ferrocene and weak O−H bonds upon activation with proton donors. Our findings provide support for the postulated mechanism of O2.− activation at class I b Mn2 RNRs.

Original languageEnglish (US)
Pages (from-to)918-922
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number4
StatePublished - Jan 22 2018


  • bioinorganic chemistry
  • dimanganese complexes
  • dioxygen/superoxide activation
  • oxidation reactions
  • ribonucleotide reductases

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    Magherusan, A. M., Zhou, A., Farquhar, E. R., García-Melchor, M., Twamley, B., Que, L., & McDonald, A. R. (2018). Mimicking Class I b Mn2-Ribonucleotide Reductase: A MnII2 Complex and Its Reaction with Superoxide. Angewandte Chemie - International Edition, 57(4), 918-922. https://doi.org/10.1002/anie.201709806