Mid-infrared spectroscopy for discrimination and classification of Aspergillus spp. contamination in peanuts

Hande Kaya-Celiker, P. Kumar Mallikarjunan, Archileo Kaaya

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this study, Aspergillus spp., common colonists in peanut, were characterized, classified and quantified using FTIR coupled with ATR accessory. FTIR-ATR spectral data of infected peanut samples were preprocessed (mean-centering, smoothing the 1st derivative), and used for the PLS regression analysis for quantitative results. Very high R2 values (96.20-99.98%) together with low error of RMSEC values (0.014-0.153 LogCFU/g of peanut) were obtained. Even, the spectrum of peanut matrix was dominant at early stages of invasion (≤2.5LogCFU/g peanut), resulting in section separation (Nigri from Flavi) and at higher population (>4LogCFU/g, species level separation (Aspergillus alliaceus, Aspergillus caelatus, Aspergillus flavus, Aspergillus parasiticus, and Aspergillus tamari) was observed. The accuracy of correct classification increased proportionally with fungal invasion level and 100% correct classification was reached when the cell level was LogCFU/g=4.5-5. Samples with similar secondary metabolites (toxin producers) grouped close-by in PC score diagrams for all levels of fungal growth. Results highlight the possible implementation of FTIR-ATR model to detect infected peanuts even at early stages of invasion; besides, to prove the potential separation capability in terms of species and their secondary metabolites.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalFood Control
Volume52
DOIs
StatePublished - Jun 1 2015

Fingerprint

infrared spectroscopy
Aspergillus
peanuts
Spectrum Analysis
Fourier Transform Infrared Spectroscopy
secondary metabolites
Aspergillus alliaceus
Aspergillus flavus
Aspergillus parasiticus
spectral analysis
microbial growth
Arachis
toxins
regression analysis
Regression Analysis
sampling
Growth
Population
cells

Keywords

  • Aspergillus species
  • Discriminant analysis
  • FTIR-ATR
  • PLS regression
  • Peanut

Cite this

Mid-infrared spectroscopy for discrimination and classification of Aspergillus spp. contamination in peanuts. / Kaya-Celiker, Hande; Mallikarjunan, P. Kumar; Kaaya, Archileo.

In: Food Control, Vol. 52, 01.06.2015, p. 103-111.

Research output: Contribution to journalArticle

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