In this study, microwave assisted puffing technology was developed to increase the extraction efficiency of polysaccharides from D. devonianum. Fresh stems of Dendrobium devonianum were processed using microwave puffing technology and the processing conditions were explored and optimized to increase the extraction yield of polysaccharides. The effects of sample length (SL), initial moisture content (IMC), microwave power (MP), and processing time (PT) on quality attributes of the products, in terms of expansion ratio and sensory evaluation, were analyzed using response surface methodology. The optimal SL, IMC, MP, and PT were 3.5 cm, 22%, 702 W, and 31 s, respectively, which resulted in optimal expansion ratio and sensory score of 326.1% and 8.63, respectively. Furthermore, the extraction yield of polysaccharides from the microwave-puffed products was increased by 18.9% compared with that from nonpuffed samples. Antioxidant and anti-hyperglycemic activities of polysaccharides from puffed and nonpuffed D. devonianum were also evaluated and compared. Practical applications: We developed a novel microwave assisted puffing technology to increase the extraction efficiency of polysaccharides from D. devonianum. Polysaccharides, the major active components of Dendrobium species, exhibit the potential as an inhibitor, including immunomodulator, antioxidant, anti-tumor and anti-hyperglycemic agent.
Bibliographical noteFunding Information:
The authors would like to thank Yuxiao Li for his technical assistance for bioactivities, teachers in Kunming University of Science and Technology for their patient assistance on the SEM experiment, the panelists for comments on the puffed products, all staffs of the laboratory for support, and the National Natural Science Foundation of China (NSFC No. 81460535, 21262047) and the Natural Science Foundation of Yunnan Province (No. S2012FZ0227) for the financial support.
National Natural Science Foundation of China, Grant/Award Numbers: NSFC No. 81460535 and 21262047; Natural Science Foundation of Yunnan Province; Grant/ Award Number: S2012FZ0227
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