Immunofluorescence microscopy of sheets of plant cells has allowed analysis of microtubule arrays across tissues. Examination of multilayer sheets with laser scanning confocal microscopy allows interior cell layers to be observed without interference from out-of-focus fluorescence. Transitory stages in preprophase band formation during stomatal complex development in grass leaves are identified, and preprophase bands are found in the relatively disorganized spongy mesophyll of spinach as well as in more highly organized mesophyll layers.
Bibliographical noteFunding Information:
We wish to thank Steve Paddock of the Integrated Microscopy Resource for Biomedical Research for his generous assistance with the confocal microscopy. The IMR in Madison is funded as an NIH Biomedical Research and Technology Resource (RR570). This work was funded also by a Grant-in-Aid from the Graduate School, University of Minnesota and by a grant from the USDA Competitive Research Program, 87-CRCR-I-2530~