Microtubule assembly dynamics: new insights at the nanoscale

Melissa K. Gardner, Alan J. Hunt, Holly V. Goodson, David J. Odde

Research output: Contribution to journalReview articlepeer-review

49 Scopus citations

Abstract

Although the dynamic self-assembly behavior of microtubule ends has been well characterized at the spatial resolution of light microscopy (∼200 nm), the single-molecule events that lead to these dynamics are less clear. Recently, a number of in vitro studies used novel approaches combining laser tweezers, microfabricated chambers, and high-resolution tracking of microtubule-bound beads to characterize mechanochemical aspects of MT dynamics at nanometer scale resolution. In addition, computational modeling is providing a framework for integrating these experimental results into physically plausible models of molecular scale microtubule dynamics. These nanoscale studies are providing new fundamental insights about microtubule assembly, and will be important for advancing our understanding of how microtubule dynamic instability is regulated in vivo via microtubule-associated proteins, therapeutic agents, and mechanical forces.

Original languageEnglish (US)
Pages (from-to)64-70
Number of pages7
JournalCurrent Opinion in Cell Biology
Volume20
Issue number1
DOIs
StatePublished - Feb 1 2008

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