Microtomographic investigation of a large corpus of cichlids

David Haberthür, Mikki Law, Kassandra Ford, Marcel Häsler, Ole Seehausen, Ruslan Hlushchuk

Research output: Contribution to journalArticlepeer-review


A large collection of cichlids (N = 133) from Lake Victoria in Africa, with total lengths ranging from 6 to 18 cm was nondestructively imaged using micro-computed tomography. We present a method to efficiently obtain three-dimensional tomographic datasets of the oral and pharyngeal jaws and the whole skull of these fishes to accurately describe their morphology. The tomographic data we acquired (9.8 TB of projection images) yielded 1.5 TB of three-dimensional image stacks used for extracting the relevant features of interest. Herein we present our method and outlooks on analyzing the acquired data; a morphological description of the oral and pharyngeal jaws, a three-dimensional geometric morphometrics analysis of landmarked skull features, and a robust method to automatically extract otoliths from the tomographic data.

Original languageEnglish (US)
Article numbere0291003
JournalPloS one
Issue number9 September
StatePublished - Sep 2023
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright: © 2023 Haberthür et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PubMed: MeSH publication types

  • Journal Article


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