Microstructural and mechanical differences between digested collagen-fibrin co-gels and pure collagen and fibrin gels

Victor K. Lai, Christina R. Frey, Allan M. Kerandi, Spencer P. Lake, Robert T. Tranquillo, Victor H. Barocas

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45 Scopus citations


Collagen and fibrin are important extracellular matrix (ECM) components in the body, providing structural integrity to various tissues. These biopolymers are also common scaffolds used in tissue engineering. This study investigated how co-gelation of collagen and fibrin affected the properties of each individual protein network. Collagen-fibrin co-gels were cast and subsequently digested using either plasmin or collagenase; the microstructure and mechanical behavior of the resulting networks were then compared with the respective pure collagen or fibrin gels of the same protein concentration. The morphologies of the collagen networks were further analyzed via three-dimensional network reconstruction from confocal image z-stacks. Both collagen and fibrin exhibited a decrease in mean fiber diameter when formed in co-gels compared with the pure gels. This microstructural change was accompanied by an increased failure strain and decreased tangent modulus for both collagen and fibrin following selective digestion of the co-gels. In addition, analysis of the reconstructed collagen networks indicated the presence of very long fibers and the clustering of fibrils, resulting in very high connectivities for collagen networks formed in co-gels.

Original languageEnglish (US)
Pages (from-to)4031-4042
Number of pages12
JournalActa Biomaterialia
Issue number11
StatePublished - Nov 2012

Bibliographical note

Funding Information:
The authors gratefully acknowledge financial support from the National Institutes of Health ( R01-EB005813 ) and the American Heart Association ( 11PRE5410003 ). We thank Guillermo Marquez of the University Imaging Center for his help with the spinning disk confocal microscope, and Dr Andy Stein for providing the FIRE code. Parts of this work were carried out in the Characterization Facility, University of Minnesota, which receives partial support from the NSF through the MRSEC program.


  • Collagen
  • Confocal microscopy
  • Fibrin
  • Mechanical properties
  • Microstructure
  • Tissue engineering


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