Microsomal phospholipase A2 is activated by surfactant toxins in 3T3 mouse fibroblasts

Kawaguchi Hideaki, Thomas W. Shier

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In 3T3 mouse fibroblasts that were treated by surfactant toxins such as staphylococcal delta toxin, melittin from bee venom and lysolecithin, microsomal phospholipase A2 (PLA2) was activated. However, these toxins did not activate PLA2 when added to cell-free homogenates or microsomal preparations. The maximal activation was achieved at 1 mM Ca2+ pH 8.5. The microsomal PLA2 stimulated by these toxins had a high fatty acid specificity (C-2 position) toward arachidonic acid.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalToxicology Letters
Volume35
Issue number2-3
DOIs
StatePublished - Feb 1987

Keywords

  • Phospholipase A
  • Swiss mouse 3T3 fibroblast
  • lysolecithin
  • melittin
  • staphylococcal delta toxin

Fingerprint

Dive into the research topics of 'Microsomal phospholipase A<sub>2</sub> is activated by surfactant toxins in 3T3 mouse fibroblasts'. Together they form a unique fingerprint.

Cite this