Microscopy investigations. Techniques, results, limitations

Bart Van Meerbeek, Marcos Vargas, Satoshi Inoue, Yasuhiro Yoshida, Jorge Perdigão, Paul Lambrechts, Guido Vanherle

Research output: Contribution to journalArticlepeer-review

119 Scopus citations


This paper critically reviews current microscopy techniques that are used to image resin-dentin interfaces and more in particular to investigate the process of hybridization and resin-tag formation. A short description of the four most commonly used microscopy techniques with their necessary specimen-preparation methodology is provided along with a discussion of their possibilities, limitations and potential artifact formation. With this critical appraisal on microscopy techniques available to study adhesive interfaces, it is hoped to encourage the use of high-resolution analytical tools to further elucidate the mechanisms of bonding at the ultra-structural level. Besides ultra-morphologic characterization, more research should be devoted to study the chemical and physical properties of resin-dentin bonds. The final objective of such fundamental research should be to establish a more durable and reliable adhesive restorative technique.

Original languageEnglish (US)
Pages (from-to)3D-18D
JournalAmerican journal of dentistry
Issue number5 SPEC. ISS.
StatePublished - Nov 1 2000


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