TY - JOUR
T1 - Microscopic analysis of fluorescence resonance energy transfer (FRET).
AU - Herman, Brian
AU - Krishnan, R. Venkata
AU - Centonze, Victoria E.
PY - 2004
Y1 - 2004
N2 - We describe here detailed practical procedures for implementing various approaches of fluorescence resonance energy transfer (FRET) in microscope-based measurements. A comprehensive theoretical formalism is developed and the different experimental procedures are outlined. A step-by-step protocol is provided for preparing the specimens for FRET measurements, data acquisition procedures, analysis, and quantification. Particular emphasis is given to exemplify the FRET applications in the study of protein-protein interactions.
AB - We describe here detailed practical procedures for implementing various approaches of fluorescence resonance energy transfer (FRET) in microscope-based measurements. A comprehensive theoretical formalism is developed and the different experimental procedures are outlined. A step-by-step protocol is provided for preparing the specimens for FRET measurements, data acquisition procedures, analysis, and quantification. Particular emphasis is given to exemplify the FRET applications in the study of protein-protein interactions.
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U2 - 10.1385/1-59259-762-9:351
DO - 10.1385/1-59259-762-9:351
M3 - Review article
C2 - 15064469
AN - SCOPUS:3242735110
SN - 1064-3745
VL - 261
SP - 351
EP - 370
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -