TY - JOUR
T1 - Microscale isolation technique for quantitative gas chromatography-mass spectrometry analysis of indole-3-acetic acid from cherry (prunus cerasus l.)
AU - Baraldi, Rita
AU - Chen, Kai Hsien
AU - Cohen, Jerry D.
PY - 1988
Y1 - 1988
N2 - A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C18 column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. 13C6indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.
AB - A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C18 column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. 13C6indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.
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U2 - 10.1016/S0021-9673(00)94477-7
DO - 10.1016/S0021-9673(00)94477-7
M3 - Article
AN - SCOPUS:45549114308
VL - 442
SP - 301
EP - 306
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -