Microsatellites from the charcoal rot fungus (Macrophomina phaseolina)

Richard E. Baird, Phillip A. Wadl, Xinwang Wang, Denita H. Johnson, Timothy A. Rinehart, Hamed K. Abbas, Thomas Shier, Robert N. Trigiano

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.

Original languageEnglish (US)
Pages (from-to)946-948
Number of pages3
JournalMolecular Ecology Resources
Volume9
Issue number3
DOIs
StatePublished - May 1 2009

Keywords

  • Genomic library
  • PCR
  • SSR enrichment

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