Microsatellite markers for the hybridizing tiger swallowtails, Papilio glaucus and P. canadensis, and their applicability to historic specimens and congeneric species

Carsten Nowak; Christian M. Brown; Jessica J. Hellmann

doi:10.1111/j.1755-0998.2008.02203.x

Microsatellite markers for the hybridizing tiger swallowtails, Papilio glaucus and P. canadensis, and their applicability to historic specimens and congeneric species

Carsten Nowak, Christian M. Brown, Jessica J. Hellmann

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We report the development of microsatellite markers for the sister species Papilio glaucus and P. canadensis (Papilionidae: Lepidoptera). All 16 markers displayed a high degree of variation in both species, ranging from eight to 24 alleles per locus. Substantial heterozygote deficits were observed for several loci, indicating the presence of null alleles. All markers were successfully used to genotype dried samples from a historical collection. Cross-species amplification with six additional Papilio species showed that most loci can be used to study genetic variation in other closely related species of tiger swallowtails.

Original language	English (US)
Pages (from-to)	800-803
Number of pages	4
Journal	Molecular Ecology Resources
Volume	9
Issue number	3
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02203.x
State	Published - May 2009
Externally published	Yes

Keywords

Canadian tiger swallowtail
Eastern tiger swallowtail
Genetic markers
Hybridization
Lepidoptera
Sister species

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10.1111/j.1755-0998.2008.02203.x

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title = "Microsatellite markers for the hybridizing tiger swallowtails, Papilio glaucus and P. canadensis, and their applicability to historic specimens and congeneric species",

abstract = "We report the development of microsatellite markers for the sister species Papilio glaucus and P. canadensis (Papilionidae: Lepidoptera). All 16 markers displayed a high degree of variation in both species, ranging from eight to 24 alleles per locus. Substantial heterozygote deficits were observed for several loci, indicating the presence of null alleles. All markers were successfully used to genotype dried samples from a historical collection. Cross-species amplification with six additional Papilio species showed that most loci can be used to study genetic variation in other closely related species of tiger swallowtails.",

keywords = "Canadian tiger swallowtail, Eastern tiger swallowtail, Genetic markers, Hybridization, Lepidoptera, Sister species",

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AU - Nowak, Carsten

AU - Brown, Christian M.

AU - Hellmann, Jessica J.

PY - 2009/5

Y1 - 2009/5

N2 - We report the development of microsatellite markers for the sister species Papilio glaucus and P. canadensis (Papilionidae: Lepidoptera). All 16 markers displayed a high degree of variation in both species, ranging from eight to 24 alleles per locus. Substantial heterozygote deficits were observed for several loci, indicating the presence of null alleles. All markers were successfully used to genotype dried samples from a historical collection. Cross-species amplification with six additional Papilio species showed that most loci can be used to study genetic variation in other closely related species of tiger swallowtails.

AB - We report the development of microsatellite markers for the sister species Papilio glaucus and P. canadensis (Papilionidae: Lepidoptera). All 16 markers displayed a high degree of variation in both species, ranging from eight to 24 alleles per locus. Substantial heterozygote deficits were observed for several loci, indicating the presence of null alleles. All markers were successfully used to genotype dried samples from a historical collection. Cross-species amplification with six additional Papilio species showed that most loci can be used to study genetic variation in other closely related species of tiger swallowtails.

KW - Canadian tiger swallowtail

KW - Eastern tiger swallowtail

KW - Genetic markers

KW - Hybridization

KW - Lepidoptera

KW - Sister species

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DO - 10.1111/j.1755-0998.2008.02203.x

M3 - Article

AN - SCOPUS:65249115884

SN - 1755-098X

VL - 9

SP - 800

EP - 803

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 3

ER -

Microsatellite markers for the hybridizing tiger swallowtails, Papilio glaucus and P. canadensis, and their applicability to historic specimens and congeneric species

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