MicroRNA miR-182 cluster mediated modulation of RECK without changes in cell surface membrane type-1 matrix metalloproteinase (MT1-MMP)

Milagros Silva, Maria E. Hernandez, Fausto Rojas, Lihua Li, Subbaya Subramanian, Michael J. Wilson

Research output: Contribution to journalArticle

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Abstract

Cell surface localized membrane type 1-matrix metalloproteinase (MT1-MMP) plays an important role in physiological and pathological processes and its function can be regulated by proteins such as RECK. We examined the ability of miR-182 (one of the miR-183 cluster miRNAs), which can target RECK, to control cell surface MT1-MMP activity. Expression of RECK mRNA and protein was increased with anti-miRs to miR-182, miR-183 or miR-96 in HT1080 fibrosarcoma cells, but, decreased RECK mRNA and increased its protein in the benign prostatic hyperplasia cell line BPH-1. Treatment of BPH-1 and HT-1080 cells with the anti-miRs did not change the level of cell surface MT1-MMP activity, nor their rate of migration in an in vitro wound-healing assay. Trichostatin A (TSA) did not increase the level of RECK, but blocked cell surface MT1-MMP activity and decreased cell motility. Anti-miRs mediated increased RECK levels did not interfere with cell surface MT1-MMP function, and TSA may block cell surface localization of MT1-MMP by a mechanism independent of RECK.

Original languageEnglish (US)
Pages (from-to)2918-2928
Number of pages11
JournalAmerican Journal of Cancer Research
Volume5
Issue number9
StatePublished - Jan 1 2015

Fingerprint

Matrix Metalloproteinase 14
Matrix Metalloproteinase 1
MicroRNAs
Cell Membrane
trichostatin A
Physiological Phenomena
Messenger RNA
Proteins
Fibrosarcoma
Prostatic Hyperplasia
Pathologic Processes
Wound Healing
Cell Movement
Cell Line
Antagomirs

Keywords

  • EMMPRIN
  • MT1-MMP
  • RECK
  • Trichostatin A (TSA)
  • miR-182
  • microRNA

Cite this

MicroRNA miR-182 cluster mediated modulation of RECK without changes in cell surface membrane type-1 matrix metalloproteinase (MT1-MMP). / Silva, Milagros; Hernandez, Maria E.; Rojas, Fausto; Li, Lihua; Subramanian, Subbaya; Wilson, Michael J.

In: American Journal of Cancer Research, Vol. 5, No. 9, 01.01.2015, p. 2918-2928.

Research output: Contribution to journalArticle

Silva, Milagros ; Hernandez, Maria E. ; Rojas, Fausto ; Li, Lihua ; Subramanian, Subbaya ; Wilson, Michael J. / MicroRNA miR-182 cluster mediated modulation of RECK without changes in cell surface membrane type-1 matrix metalloproteinase (MT1-MMP). In: American Journal of Cancer Research. 2015 ; Vol. 5, No. 9. pp. 2918-2928.
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