MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines

Lynne T. Bemis, Robert Chen, Carol M. Amato, Elizabeth H. Classen, Steven E. Robinson, David G. Coffey, Paul F. Erickson, Yiqun G. Shellman, William A. Robinson

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Micropthalmia-associated transcription factor (MITF) is the master regulator of melanocyte development, survival, and function. Frequent alteration in the expression of MITF is detected in melanoma, but the mechanism(s) underlying the alteration in expression have not been completely determined. In these studies, we have identified microRNA-137 (miR-137) as a regulator of MITF expression. The genomic locus of miR-137 at chromosome 1p22 places it in a region of the human genome previously determined to harbor an allele for melanoma susceptibility. Here, we show that expression of mature miR-137 in melanoma cell lines down-regulates MITF expression. Further, we have identified a 15-bp variable nucleotide tandem repeat located just 5′ to the pre-miR-137 sequence, which alters the processing and function of miR-137 in melanoma cell lines.

Original languageEnglish (US)
Pages (from-to)1362-1368
Number of pages7
JournalCancer Research
Volume68
Issue number5
DOIs
StatePublished - Mar 1 2008

Fingerprint

Microphthalmia-Associated Transcription Factor
MicroRNAs
Melanoma
Cell Line
Transcription Factors
Minisatellite Repeats
Melanocytes
Human Genome
Down-Regulation
Nucleotides
Chromosomes
Alleles

Cite this

Bemis, L. T., Chen, R., Amato, C. M., Classen, E. H., Robinson, S. E., Coffey, D. G., ... Robinson, W. A. (2008). MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines. Cancer Research, 68(5), 1362-1368. https://doi.org/10.1158/0008-5472.CAN-07-2912

MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines. / Bemis, Lynne T.; Chen, Robert; Amato, Carol M.; Classen, Elizabeth H.; Robinson, Steven E.; Coffey, David G.; Erickson, Paul F.; Shellman, Yiqun G.; Robinson, William A.

In: Cancer Research, Vol. 68, No. 5, 01.03.2008, p. 1362-1368.

Research output: Contribution to journalArticle

Bemis, LT, Chen, R, Amato, CM, Classen, EH, Robinson, SE, Coffey, DG, Erickson, PF, Shellman, YG & Robinson, WA 2008, 'MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines', Cancer Research, vol. 68, no. 5, pp. 1362-1368. https://doi.org/10.1158/0008-5472.CAN-07-2912
Bemis, Lynne T. ; Chen, Robert ; Amato, Carol M. ; Classen, Elizabeth H. ; Robinson, Steven E. ; Coffey, David G. ; Erickson, Paul F. ; Shellman, Yiqun G. ; Robinson, William A. / MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines. In: Cancer Research. 2008 ; Vol. 68, No. 5. pp. 1362-1368.
@article{56d23547a3874b22acdc7bea5f60ac16,
title = "MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines",
abstract = "Micropthalmia-associated transcription factor (MITF) is the master regulator of melanocyte development, survival, and function. Frequent alteration in the expression of MITF is detected in melanoma, but the mechanism(s) underlying the alteration in expression have not been completely determined. In these studies, we have identified microRNA-137 (miR-137) as a regulator of MITF expression. The genomic locus of miR-137 at chromosome 1p22 places it in a region of the human genome previously determined to harbor an allele for melanoma susceptibility. Here, we show that expression of mature miR-137 in melanoma cell lines down-regulates MITF expression. Further, we have identified a 15-bp variable nucleotide tandem repeat located just 5′ to the pre-miR-137 sequence, which alters the processing and function of miR-137 in melanoma cell lines.",
author = "Bemis, {Lynne T.} and Robert Chen and Amato, {Carol M.} and Classen, {Elizabeth H.} and Robinson, {Steven E.} and Coffey, {David G.} and Erickson, {Paul F.} and Shellman, {Yiqun G.} and Robinson, {William A.}",
year = "2008",
month = "3",
day = "1",
doi = "10.1158/0008-5472.CAN-07-2912",
language = "English (US)",
volume = "68",
pages = "1362--1368",
journal = "Cancer Research",
issn = "0008-5472",
number = "5",

}

TY - JOUR

T1 - MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines

AU - Bemis, Lynne T.

AU - Chen, Robert

AU - Amato, Carol M.

AU - Classen, Elizabeth H.

AU - Robinson, Steven E.

AU - Coffey, David G.

AU - Erickson, Paul F.

AU - Shellman, Yiqun G.

AU - Robinson, William A.

PY - 2008/3/1

Y1 - 2008/3/1

N2 - Micropthalmia-associated transcription factor (MITF) is the master regulator of melanocyte development, survival, and function. Frequent alteration in the expression of MITF is detected in melanoma, but the mechanism(s) underlying the alteration in expression have not been completely determined. In these studies, we have identified microRNA-137 (miR-137) as a regulator of MITF expression. The genomic locus of miR-137 at chromosome 1p22 places it in a region of the human genome previously determined to harbor an allele for melanoma susceptibility. Here, we show that expression of mature miR-137 in melanoma cell lines down-regulates MITF expression. Further, we have identified a 15-bp variable nucleotide tandem repeat located just 5′ to the pre-miR-137 sequence, which alters the processing and function of miR-137 in melanoma cell lines.

AB - Micropthalmia-associated transcription factor (MITF) is the master regulator of melanocyte development, survival, and function. Frequent alteration in the expression of MITF is detected in melanoma, but the mechanism(s) underlying the alteration in expression have not been completely determined. In these studies, we have identified microRNA-137 (miR-137) as a regulator of MITF expression. The genomic locus of miR-137 at chromosome 1p22 places it in a region of the human genome previously determined to harbor an allele for melanoma susceptibility. Here, we show that expression of mature miR-137 in melanoma cell lines down-regulates MITF expression. Further, we have identified a 15-bp variable nucleotide tandem repeat located just 5′ to the pre-miR-137 sequence, which alters the processing and function of miR-137 in melanoma cell lines.

UR - http://www.scopus.com/inward/record.url?scp=40449094344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40449094344&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-07-2912

DO - 10.1158/0008-5472.CAN-07-2912

M3 - Article

C2 - 18316599

AN - SCOPUS:40449094344

VL - 68

SP - 1362

EP - 1368

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 5

ER -