MicroRNA-137 targets carboxyl-terminal binding protein 1 in melanoma cell lines

Yu Deng, Hui Deng, Feng Bi, Jing Liu, Lynn T. Bemis, David Norris, Xiao Jing Wang, Qinghong Zhang

Research output: Contribution to journalArticlepeer-review

67 Scopus citations


Carboxyl-terminal binding protein 1 (CtBP1) is a transcriptional co-repressor that represses expression of various tumor suppressor genes. In the present study, we identified miR-137 as a potential regulator of CtBP1 expression in melanoma cells. Expression of miR-137 in melanoma cell lines was found to inversely correlate with CtBP1 levels. Target Scan predicted a putative site for miR-137 within the CtBP1 3′ untranslated region (3′UTR) at nt 710-716, which is highly conserved across species. To explore the mechanism of miR-137 targeting CtBP1, we performed an Argonaute 2 (Ago2)-pull down assay, and miR-137 was identified in complex with CtBP1 mRNA. miR-137 suppressed CtBP1 3′ UTR luciferase-reporter activity, and this effect was lost with deletion of the putative 3′ UTR target-site. Consistent with the results of the reporter assay, ectopic expression of miR-137 reduced expression levels of CtBP1. Furthermore, expression of miR-137 increased the immediate downstream effectors of CtBP1, such as E-cadherin and Bax. The human miR-137 gene is located at chromosome 1p22, which has previously been determined to be a susceptive region for melanoma. This study suggests miR-137 may act as a tumor suppressor by directly targeting CtBP1 to inhibit epithelial-mesenchymal transition (EMT) and inducing apoptosis of melanoma cells, thus illustrating a functional link between miR-137 and CtBP1 in melanoma development

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalInternational Journal of Biological Sciences
Issue number1
StatePublished - 2011
Externally publishedYes


  • CtBP1
  • Melanoma
  • Transcription
  • Tumor suppressor
  • miR-137


Dive into the research topics of 'MicroRNA-137 targets carboxyl-terminal binding protein 1 in melanoma cell lines'. Together they form a unique fingerprint.

Cite this