Micropipette aspiration of the Pacinian corpuscle

Julia C. Quindlen, Ellen T. Bloom, Laura E. Ortega, Amy Moeller, Victor H Barocas

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The Pacinian corpuscle (PC) is a cutaneous mechanoreceptor sensitive to high-frequency vibrations (20–1000 Hz). The PC is of importance due to its integral role in somatosensation and the critical need to understand PC function for haptic feedback system development. Previous theoretical and computational studies have modeled the physiological response of the PC to sustained or vibrating mechanical stimuli, but they have used estimates of the receptor's mechanical properties, which remain largely unmeasured. In this study, we used micropipette aspiration (MPA) to determine an apparent Young's modulus for PCs isolated from a cadaveric human hand. MPA was applied in increments of 5 mm H2O (49 Pa), and the change in protrusion length of the PC into the pipette was recorded. The protrusion length vs. suction pressure data was used to calculate the apparent Young's modulus. Using 10 PCs with long-axis lengths of 2.99 mm ± 0.41 mm and short-axis lengths of 1.45 mm ± 0.22 mm, we calculated a Young's modulus of 1.40 ± 0.86 kPa. Our measurement is on the same order of magnitude as those approximated in previous models, which estimated the PC to be on the same order of magnitude as skin or isolated cells, so we recommend that a modulus in the kPa range be used in future studies.

Original languageEnglish (US)
Pages (from-to)104-109
Number of pages6
JournalJournal of Biomechanics
Volume63
DOIs
StatePublished - Oct 3 2017

Fingerprint

Pacinian Corpuscles
Elastic moduli
Elastic Modulus
Skin
Feedback
Mechanical properties
Mechanoreceptors
Suction
Vibration
Theoretical Models
Hand
Pressure

Keywords

  • Biomechanics
  • Haptics
  • Neuroscience
  • Touch

Cite this

Micropipette aspiration of the Pacinian corpuscle. / Quindlen, Julia C.; Bloom, Ellen T.; Ortega, Laura E.; Moeller, Amy; Barocas, Victor H.

In: Journal of Biomechanics, Vol. 63, 03.10.2017, p. 104-109.

Research output: Contribution to journalArticle

Quindlen, Julia C. ; Bloom, Ellen T. ; Ortega, Laura E. ; Moeller, Amy ; Barocas, Victor H. / Micropipette aspiration of the Pacinian corpuscle. In: Journal of Biomechanics. 2017 ; Vol. 63. pp. 104-109.
@article{13d2b6bced3f41dfb1e8ddacf8b6e1b9,
title = "Micropipette aspiration of the Pacinian corpuscle",
abstract = "The Pacinian corpuscle (PC) is a cutaneous mechanoreceptor sensitive to high-frequency vibrations (20–1000 Hz). The PC is of importance due to its integral role in somatosensation and the critical need to understand PC function for haptic feedback system development. Previous theoretical and computational studies have modeled the physiological response of the PC to sustained or vibrating mechanical stimuli, but they have used estimates of the receptor's mechanical properties, which remain largely unmeasured. In this study, we used micropipette aspiration (MPA) to determine an apparent Young's modulus for PCs isolated from a cadaveric human hand. MPA was applied in increments of 5 mm H2O (49 Pa), and the change in protrusion length of the PC into the pipette was recorded. The protrusion length vs. suction pressure data was used to calculate the apparent Young's modulus. Using 10 PCs with long-axis lengths of 2.99 mm ± 0.41 mm and short-axis lengths of 1.45 mm ± 0.22 mm, we calculated a Young's modulus of 1.40 ± 0.86 kPa. Our measurement is on the same order of magnitude as those approximated in previous models, which estimated the PC to be on the same order of magnitude as skin or isolated cells, so we recommend that a modulus in the kPa range be used in future studies.",
keywords = "Biomechanics, Haptics, Neuroscience, Touch",
author = "Quindlen, {Julia C.} and Bloom, {Ellen T.} and Ortega, {Laura E.} and Amy Moeller and Barocas, {Victor H}",
year = "2017",
month = "10",
day = "3",
doi = "10.1016/j.jbiomech.2017.08.005",
language = "English (US)",
volume = "63",
pages = "104--109",
journal = "Journal of Biomechanics",
issn = "0021-9290",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Micropipette aspiration of the Pacinian corpuscle

AU - Quindlen, Julia C.

AU - Bloom, Ellen T.

AU - Ortega, Laura E.

AU - Moeller, Amy

AU - Barocas, Victor H

PY - 2017/10/3

Y1 - 2017/10/3

N2 - The Pacinian corpuscle (PC) is a cutaneous mechanoreceptor sensitive to high-frequency vibrations (20–1000 Hz). The PC is of importance due to its integral role in somatosensation and the critical need to understand PC function for haptic feedback system development. Previous theoretical and computational studies have modeled the physiological response of the PC to sustained or vibrating mechanical stimuli, but they have used estimates of the receptor's mechanical properties, which remain largely unmeasured. In this study, we used micropipette aspiration (MPA) to determine an apparent Young's modulus for PCs isolated from a cadaveric human hand. MPA was applied in increments of 5 mm H2O (49 Pa), and the change in protrusion length of the PC into the pipette was recorded. The protrusion length vs. suction pressure data was used to calculate the apparent Young's modulus. Using 10 PCs with long-axis lengths of 2.99 mm ± 0.41 mm and short-axis lengths of 1.45 mm ± 0.22 mm, we calculated a Young's modulus of 1.40 ± 0.86 kPa. Our measurement is on the same order of magnitude as those approximated in previous models, which estimated the PC to be on the same order of magnitude as skin or isolated cells, so we recommend that a modulus in the kPa range be used in future studies.

AB - The Pacinian corpuscle (PC) is a cutaneous mechanoreceptor sensitive to high-frequency vibrations (20–1000 Hz). The PC is of importance due to its integral role in somatosensation and the critical need to understand PC function for haptic feedback system development. Previous theoretical and computational studies have modeled the physiological response of the PC to sustained or vibrating mechanical stimuli, but they have used estimates of the receptor's mechanical properties, which remain largely unmeasured. In this study, we used micropipette aspiration (MPA) to determine an apparent Young's modulus for PCs isolated from a cadaveric human hand. MPA was applied in increments of 5 mm H2O (49 Pa), and the change in protrusion length of the PC into the pipette was recorded. The protrusion length vs. suction pressure data was used to calculate the apparent Young's modulus. Using 10 PCs with long-axis lengths of 2.99 mm ± 0.41 mm and short-axis lengths of 1.45 mm ± 0.22 mm, we calculated a Young's modulus of 1.40 ± 0.86 kPa. Our measurement is on the same order of magnitude as those approximated in previous models, which estimated the PC to be on the same order of magnitude as skin or isolated cells, so we recommend that a modulus in the kPa range be used in future studies.

KW - Biomechanics

KW - Haptics

KW - Neuroscience

KW - Touch

UR - http://www.scopus.com/inward/record.url?scp=85028332619&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028332619&partnerID=8YFLogxK

U2 - 10.1016/j.jbiomech.2017.08.005

DO - 10.1016/j.jbiomech.2017.08.005

M3 - Article

VL - 63

SP - 104

EP - 109

JO - Journal of Biomechanics

JF - Journal of Biomechanics

SN - 0021-9290

ER -