Micrometer-scale membrane transition of supported lipid bilayer membrane reconstituted with cytosol of Dictyostelium discoideum

Kei Takahashi, Taro Toyota

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background: The transformation of the supported lipid bilayer (SLB) membrane by extracted cytosol from living resources, has recently drawn much attention. It enables us to address the question of whether the purified phospholipid SLB membrane, including lipids related to amoeba locomotion, which was discussed in many previous studies, exhibits membrane deformation in the presence of cytosol extracted from amoeba; Methods: In this report, a method for reconstituting a supported lipid bilayer (SLB) membrane, composed of purified phospholipids and cytosol extracted from Dictyostelium discoideum, is described. This technique is a new reconstitution method combining the artificial constitution of membranes with the reconstitution using animate cytosol (without precise purification at a molecular level), contributing to membrane deformation analysis; Results: The morphology transition of a SLB membrane composed of phosphatidylcholines, after the addition of cytosolic extract, was traced using a confocal laser scanning fluorescence microscope. As a result, pore formation in the SLB membrane was observed and phosphatidylinositides incorporated into the SLB membrane tended to suppress pore formation and expansion; Conclusions: The current findings imply that phosphatidylinositides have the potential to control cytoplasm activity and bind to a phosphoinositide-containing SLB membrane.

Original languageEnglish (US)
Article number11
JournalLife
Volume7
Issue number1
DOIs
StatePublished - Mar 2017

Bibliographical note

Publisher Copyright:
© 2017 by the authors.

Keywords

  • Cytosol
  • Dictyostelium discoideum
  • Phosphatidylinositides
  • Supported lipid bilayer membrane

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