Micromachined broadband RF cytometer for high-throughput analysis of mammalian cells

Sang Hyun Oh, David K. Wood, Sang Ho Lee, Karen Y. Dane, Patrick S. Daugherty, H. T. Soh, A. N. Cleland

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

We have microfabricated a continuous-flow, high-bandwidth Coulter counter that uses a radiofrequency (RF) probe to measure RF reflectance from biological cells in the microfluidic channel. This scheme eliminates the trade-off between detection sensitivity and throughput, which exists in previous approaches. Using this technique, we have demonstrated the fastest microfluidic cytometer reported to date, which operates at 240 MHz and has intrinsic detection rates near 10 MHz. We also present real-time detection data on human breast tumor cells.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1507-1509
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • Cell impedance
  • Coulter counter
  • Cytometer
  • MEMS

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    Oh, S. H., Wood, D. K., Lee, S. H., Dane, K. Y., Daugherty, P. S., Soh, H. T., & Cleland, A. N. (2005). Micromachined broadband RF cytometer for high-throughput analysis of mammalian cells. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1507-1509). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.