Microgram-scale protein structure determination by NMR

James M. Aramini, Paolo Rossi, Clemens Anklin, Rong Xiao, Gaetano T. Montelione

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 μg (6 μl, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.

Original languageEnglish (US)
Pages (from-to)491-493
Number of pages3
JournalNature Methods
Volume4
Issue number6
DOIs
StatePublished - Jun 2007

Bibliographical note

Funding Information:
We thank T. Acton for helpful discussions. This work was supported by a grant from the Protein Structure Initiative of the US National Institutes of Health (U54 GM074958).

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