Microglial derived nitric oxide decreases serotonin content in rat basophilic leukemia (RBL-2H3) cells

David R. Linden; Esam E El-Fakahany

doi:10.1016/S0014-2999(01)01615-6

Microglial derived nitric oxide decreases serotonin content in rat basophilic leukemia (RBL-2H3) cells

David R. Linden, Esam E El-Fakahany

Experimental and Clinical Pharmacology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Nitric oxide (NO) and serotonin (5-hydroxytryptamine; 5-HT) are important neuromodulators that are involved in a myriad of biochemical reactions. In this work, we describe a novel model co-culture system to study the interactions between NO and 5-HT. NO derived from cytokine stimulated Bv2 microglial cells depleted 5-HT from RBL-2H3 cells. Reduction of 5-HT content by NO derived from the NO donor S-nitroso-N-acetylpenicillamine (SNAP) was concentration-dependent, independent of intracellular Ca²⁺ and inhibited by reduced glutathione (GSH). Collectively, these data indicate that this cell co-culture system is a viable model to study the mechanisms of interaction between nitrergic and serotonergic pathways.

Original language	English (US)
Pages (from-to)	53-56
Number of pages	4
Journal	European Journal of Pharmacology
Volume	436
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-2999(01)01615-6
State	Published - Feb 1 2002

Bibliographical note

Funding Information:
The authors would like to thank Ms. Kristin Schreiber for her valuable input to the development of these studies. This work was supported by the NIH grant NS25743.

Keywords

5-HT (5-hydroxytryptamine, serotonin)
Neuromodulation
Nitric oxide (NO) synthase

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0014-2999(01)01615-6

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title = "Microglial derived nitric oxide decreases serotonin content in rat basophilic leukemia (RBL-2H3) cells",

abstract = "Nitric oxide (NO) and serotonin (5-hydroxytryptamine; 5-HT) are important neuromodulators that are involved in a myriad of biochemical reactions. In this work, we describe a novel model co-culture system to study the interactions between NO and 5-HT. NO derived from cytokine stimulated Bv2 microglial cells depleted 5-HT from RBL-2H3 cells. Reduction of 5-HT content by NO derived from the NO donor S-nitroso-N-acetylpenicillamine (SNAP) was concentration-dependent, independent of intracellular Ca2+ and inhibited by reduced glutathione (GSH). Collectively, these data indicate that this cell co-culture system is a viable model to study the mechanisms of interaction between nitrergic and serotonergic pathways.",

keywords = "5-HT (5-hydroxytryptamine, serotonin), Neuromodulation, Nitric oxide (NO) synthase",

author = "Linden, {David R.} and El-Fakahany, {Esam E}",

note = "Funding Information: The authors would like to thank Ms. Kristin Schreiber for her valuable input to the development of these studies. This work was supported by the NIH grant NS25743.",

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AU - Linden, David R.

AU - El-Fakahany, Esam E

N1 - Funding Information: The authors would like to thank Ms. Kristin Schreiber for her valuable input to the development of these studies. This work was supported by the NIH grant NS25743.

PY - 2002/2/1

Y1 - 2002/2/1

N2 - Nitric oxide (NO) and serotonin (5-hydroxytryptamine; 5-HT) are important neuromodulators that are involved in a myriad of biochemical reactions. In this work, we describe a novel model co-culture system to study the interactions between NO and 5-HT. NO derived from cytokine stimulated Bv2 microglial cells depleted 5-HT from RBL-2H3 cells. Reduction of 5-HT content by NO derived from the NO donor S-nitroso-N-acetylpenicillamine (SNAP) was concentration-dependent, independent of intracellular Ca2+ and inhibited by reduced glutathione (GSH). Collectively, these data indicate that this cell co-culture system is a viable model to study the mechanisms of interaction between nitrergic and serotonergic pathways.

AB - Nitric oxide (NO) and serotonin (5-hydroxytryptamine; 5-HT) are important neuromodulators that are involved in a myriad of biochemical reactions. In this work, we describe a novel model co-culture system to study the interactions between NO and 5-HT. NO derived from cytokine stimulated Bv2 microglial cells depleted 5-HT from RBL-2H3 cells. Reduction of 5-HT content by NO derived from the NO donor S-nitroso-N-acetylpenicillamine (SNAP) was concentration-dependent, independent of intracellular Ca2+ and inhibited by reduced glutathione (GSH). Collectively, these data indicate that this cell co-culture system is a viable model to study the mechanisms of interaction between nitrergic and serotonergic pathways.

KW - 5-HT (5-hydroxytryptamine, serotonin)

KW - Neuromodulation

KW - Nitric oxide (NO) synthase

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UR - http://www.scopus.com/inward/citedby.url?scp=0036468372&partnerID=8YFLogxK

U2 - 10.1016/S0014-2999(01)01615-6

DO - 10.1016/S0014-2999(01)01615-6

M3 - Article

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AN - SCOPUS:0036468372

SN - 0014-2999

VL - 436

SP - 53

EP - 56

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 1-2

ER -

Microglial derived nitric oxide decreases serotonin content in rat basophilic leukemia (RBL-2H3) cells

Abstract

Bibliographical note

Keywords

UN SDGs

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