Systèmes microfluidiques de particules magnétiques auto-assemblées; application à la séparation d'ADN et à la digestion de protéines

Nicolas Minc; Marcela Slovakova; Kevin D. Dorfman; Claus Fütterer; Plamen Bokov; Zuzana Bilkova; Claire Smadga; Myriam Taverna; Jean Louis Viovy

doi:10.1051/lhb:200604007

Systèmes microfluidiques de particules magnétiques auto-assemblées; application à la séparation d'ADN et à la digestion de protéines

Translated title of the contribution: Microfluidic systems of self-assembled magnetic particles; application to DNA separation and protein digestion

Nicolas Minc
, Marcela Slovakova
, Kevin D. Dorfman
, Claus Fütterer
, Plamen Bokov
, Zuzana Bilkova
, Claire Smadga
, Myriam Taverna
, Jean Louis Viovy

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We present two experiments of bioanalysis based on the self-organization of magnetic beads under a magnetic field, realized microfluidic chips, In the first one, the beads are organized in a quasi-hexagonal array of columns used as a matrix to achieve and study the separation of long fragments of DNA. In the second one, trypsin is covalently grafted onto beads that are immobilized in a microchannel, and different proteins are then digested through the bead plug. The products of digestion are then analyzed hy MS or CE.

Translated title of the contribution	Microfluidic systems of self-assembled magnetic particles; application to DNA separation and protein digestion
Original language	French
Pages (from-to)	51-54
Number of pages	4
Journal	Houille Blanche
Issue number	4
DOIs	https://doi.org/10.1051/lhb:200604007
State	Published - 2006

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10.1051/lhb:200604007

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@article{fd05a54f885144cc8a18190dbc120503,

title = "Syst{\`e}mes microfluidiques de particules magn{\'e}tiques auto-assembl{\'e}es; application {\`a} la s{\'e}paration d'ADN et {\`a} la digestion de prot{\'e}ines",

abstract = "We present two experiments of bioanalysis based on the self-organization of magnetic beads under a magnetic field, realized microfluidic chips, In the first one, the beads are organized in a quasi-hexagonal array of columns used as a matrix to achieve and study the separation of long fragments of DNA. In the second one, trypsin is covalently grafted onto beads that are immobilized in a microchannel, and different proteins are then digested through the bead plug. The products of digestion are then analyzed hy MS or CE.",

author = "Nicolas Minc and Marcela Slovakova and Dorfman, \{Kevin D.\} and Claus F{\"u}tterer and Plamen Bokov and Zuzana Bilkova and Claire Smadga and Myriam Taverna and Viovy, \{Jean Louis\}",

year = "2006",

doi = "10.1051/lhb:200604007",

language = "French",

pages = "51--54",

journal = "Houille Blanche",

issn = "0018-6368",

publisher = "Taylor and Francis Ltd.",

number = "4",

}

TY - JOUR

T1 - Systèmes microfluidiques de particules magnétiques auto-assemblées; application à la séparation d'ADN et à la digestion de protéines

AU - Minc, Nicolas

AU - Slovakova, Marcela

AU - Dorfman, Kevin D.

AU - Fütterer, Claus

AU - Bokov, Plamen

AU - Bilkova, Zuzana

AU - Smadga, Claire

AU - Taverna, Myriam

AU - Viovy, Jean Louis

PY - 2006

Y1 - 2006

N2 - We present two experiments of bioanalysis based on the self-organization of magnetic beads under a magnetic field, realized microfluidic chips, In the first one, the beads are organized in a quasi-hexagonal array of columns used as a matrix to achieve and study the separation of long fragments of DNA. In the second one, trypsin is covalently grafted onto beads that are immobilized in a microchannel, and different proteins are then digested through the bead plug. The products of digestion are then analyzed hy MS or CE.

AB - We present two experiments of bioanalysis based on the self-organization of magnetic beads under a magnetic field, realized microfluidic chips, In the first one, the beads are organized in a quasi-hexagonal array of columns used as a matrix to achieve and study the separation of long fragments of DNA. In the second one, trypsin is covalently grafted onto beads that are immobilized in a microchannel, and different proteins are then digested through the bead plug. The products of digestion are then analyzed hy MS or CE.

UR - https://www.scopus.com/pages/publications/33749077703

UR - https://www.scopus.com/pages/publications/33749077703#tab=citedBy

U2 - 10.1051/lhb:200604007

DO - 10.1051/lhb:200604007

M3 - Article

AN - SCOPUS:33749077703

SN - 0018-6368

SP - 51

EP - 54

JO - Houille Blanche

JF - Houille Blanche

IS - 4

ER -

Systèmes microfluidiques de particules magnétiques auto-assemblées; application à la séparation d'ADN et à la digestion de protéines

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