Microfluidic sorting of microtissues

D. G. Buschke, P. Resto, N. Schumacher, B. Cox, A. Tallavajhula, A. Vivekanandan, K. W. Eliceiri, J. C. Williams, B. M. Ogle

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Increasingly, invitro culture of adherent cell types utilizes three-dimensional (3D) scaffolds or aggregate culture strategies to mimic tissue-like, microenvironmental conditions. In parallel, new flow cytometry-based technologies are emerging to accurately analyze the composition and function of these microtissues (i.e., large particles) in a non-invasive and high-throughput way. Lacking, however, is an accessible platform that can be used to effectively sort or purify large particles based on analysis parameters. Here we describe a microfluidic-based, electromechanical approach to sort large particles. Specifically, sheath-less asymmetric curving channels were employed to separate and hydrodynamically focus particles to be analyzed and subsequently sorted. This design was developed and characterized based on wall shear stress, tortuosity of the flow path, vorticity of the fluid in the channel, sorting efficiency and enrichment ratio. The large particle sorting device was capable of purifying fluorescently labelled embryoid bodies (EBs) from unlabelled EBs with an efficiency of 87.3% ± 13.5%, and enrichment ratio of 12.2 ± 8.4 (n = 8), while preserving cell viability, differentiation potential, and long-term function.

Original languageEnglish (US)
Article number014116
JournalBiomicrofluidics
Volume6
Issue number1
DOIs
StatePublished - Mar 2 2012

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Embryoid Bodies
Microfluidics
classifying
Sorting
Parallel flow
Flow cytometry
Vorticity
Scaffolds
Shear stress
Cell Differentiation
Cell Survival
Flow Cytometry
Cell Culture Techniques
Cells
Throughput
Tissue
Technology
Equipment and Supplies
Fluids
Chemical analysis

Cite this

Buschke, D. G., Resto, P., Schumacher, N., Cox, B., Tallavajhula, A., Vivekanandan, A., ... Ogle, B. M. (2012). Microfluidic sorting of microtissues. Biomicrofluidics, 6(1), [014116]. https://doi.org/10.1063/1.3692765

Microfluidic sorting of microtissues. / Buschke, D. G.; Resto, P.; Schumacher, N.; Cox, B.; Tallavajhula, A.; Vivekanandan, A.; Eliceiri, K. W.; Williams, J. C.; Ogle, B. M.

In: Biomicrofluidics, Vol. 6, No. 1, 014116, 02.03.2012.

Research output: Contribution to journalArticle

Buschke, DG, Resto, P, Schumacher, N, Cox, B, Tallavajhula, A, Vivekanandan, A, Eliceiri, KW, Williams, JC & Ogle, BM 2012, 'Microfluidic sorting of microtissues', Biomicrofluidics, vol. 6, no. 1, 014116. https://doi.org/10.1063/1.3692765
Buschke DG, Resto P, Schumacher N, Cox B, Tallavajhula A, Vivekanandan A et al. Microfluidic sorting of microtissues. Biomicrofluidics. 2012 Mar 2;6(1). 014116. https://doi.org/10.1063/1.3692765
Buschke, D. G. ; Resto, P. ; Schumacher, N. ; Cox, B. ; Tallavajhula, A. ; Vivekanandan, A. ; Eliceiri, K. W. ; Williams, J. C. ; Ogle, B. M. / Microfluidic sorting of microtissues. In: Biomicrofluidics. 2012 ; Vol. 6, No. 1.
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