Microfluidic methods for aptamer selection and characterization

Sean K. Dembowski, Michael T. Bowser

Research output: Contribution to journalReview articlepeer-review

25 Scopus citations

Abstract

Nucleic acid aptamers have tremendous potential as molecular recognition elements in biomedical targeting, analytical arrays, and self-signaling sensors. However, practical limitations and inefficiencies in the process of selecting novel aptamers (SELEX) have hampered widespread adoption of aptamer technologies. Many factors have recently contributed to more effective aptamer screening, but no influence has done more to increase the efficiency, scale, and automation of aptamer selection than that of new microfluidic SELEX techniques. This review introduces aptamers as a powerful chemical and biological tool, briefly highlights traditional SELEX techniques and their limitations, covers in detail the recent advancements in microfluidic methods of aptamer selection and characterization, and suggests possible future directions of the field.

Original languageEnglish (US)
Pages (from-to)21-32
Number of pages12
JournalAnalyst
Volume143
Issue number1
DOIs
StatePublished - Jan 7 2018

Bibliographical note

Funding Information:
The authors gratefully acknowledge funding from the National Institutes of Health (R01 GM063533).

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

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