Microfluidic KIT-ON-A-LID: A versatile platform for neutrophil chemotaxis assays and asthma diagnostics

Eric K. Sackmann, Erwin Berthier, Edmond W.K. Young, Miriam A. Shelef, Paul Fichtinger, Elizabeth Schwantes, Mike Evans, Sameer Mathur, Anna Huttenlocher, David J. Beebe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Improvements in neutrophil cliemotaxis assays have enabled significant advances in understanding the mechanisms of neutrophil recruitment, however current methods are still limiting due to high sample volume requirements, low experimental throughput, or complex assay protocols. We report a microfluidic technology that performs neutrophil sorting and cliemotaxis on-chip within minutes using nanoliters of whole blood and only requires a micropipette to operate. The platform was adapted to incorporate an endothelial cell monolayer: perform cliemotaxis on mouse neutrophils; and human neutrophil cliemotaxis in 3D. Finally, the platform was employed in a clinical setting to diagnose asthma in human patients.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages216-218
Number of pages3
ISBN (Print)9780979806452
StatePublished - Jan 1 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period10/28/1211/1/12

Keywords

  • Asthma diagnostics
  • Feno
  • Microfluidics
  • Neutrophil cliemotaxis
  • Passive pumping

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