@inproceedings{3c9d437bac044ec7a8435d8a2dfd311e,
title = "Microfluidic KIT-ON-A-LID: A versatile platform for neutrophil chemotaxis assays and asthma diagnostics",
abstract = "Improvements in neutrophil cliemotaxis assays have enabled significant advances in understanding the mechanisms of neutrophil recruitment, however current methods are still limiting due to high sample volume requirements, low experimental throughput, or complex assay protocols. We report a microfluidic technology that performs neutrophil sorting and cliemotaxis on-chip within minutes using nanoliters of whole blood and only requires a micropipette to operate. The platform was adapted to incorporate an endothelial cell monolayer: perform cliemotaxis on mouse neutrophils; and human neutrophil cliemotaxis in 3D. Finally, the platform was employed in a clinical setting to diagnose asthma in human patients.",
keywords = "Asthma diagnostics, Feno, Microfluidics, Neutrophil cliemotaxis, Passive pumping",
author = "Sackmann, {Eric K.} and Erwin Berthier and Young, {Edmond W.K.} and Shelef, {Miriam A.} and Paul Fichtinger and Elizabeth Schwantes and Mike Evans and Sameer Mathur and Anna Huttenlocher and Beebe, {David J.}",
year = "2012",
month = jan,
day = "1",
language = "English (US)",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",
pages = "216--218",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}
