Abstract
Supported or modified enzymes in the form of mobile nanoparticles are designed for enhanced activities and stabilities; however, their practical operations are dwarfed due to their tiny size which always makes recycling an arduous task and a potential risk to the environment. To overcome such drawbacks, this chapter describes a method for the preparation of a new form of microcapsules, possessing single-cavity compartments and nano-pores in the shell, to encage nanoparticle-based biocatalysts and form cell-like microreactors (CLMRs). The encaged nanoscale catalysts are maintained their high activities as in a bulk-phase solution, while they could be handled as materials of sizes hundreds-fold larger.
Original language | English (US) |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 161-174 |
Number of pages | 14 |
DOIs | |
State | Published - 2011 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 743 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© 2011, Springer Science+Business Media, LLC.
Keywords
- Encapsulation
- double emulsion
- enzyme immobilization
- hierarchical structure
- nanoparticles