Microdissection of the Y chromosome and fluorescence in situ hybridization analysis of the sex chromosomes of lake trout, Salvelinus namaycush

K. M. Reed, S. K. Bohlander, R. B. Phillips

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Lake trout, Salvelinus namaycush, is one of the few salmonids with morphologically differentiated sex chromosomes. Genetic analysis suggested that the sex-determining region of this species lies on the short arm of the Y chromosome. The differential arm of the Y chromosome was microdissected and the resulting DNA amplified in a sequence-independent manner. Amplified DNA was biotin labeled as a probe for fluorescence in situ hybridization (FISH). Strong hybridization signals were seen covering defined regions of both the Y and X chromosomes. Homeologous chromosomes of the ancestrally tetraploid genome were not identified by FISH with the Y probe, indicating diploidization of this region of the genome.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalChromosome Research
Volume3
Issue number4
DOIs
StatePublished - Jun 1 1995

Keywords

  • fluorescence in situ hybridization
  • lake trout
  • microdissection
  • sex chromosomes

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