Microculture method for mixed lymphocyte cultures in the horse.

J. J. McClure, C. C. Muscoplat, D. W. Johnson, D. R. Senogles

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A miniaturized method for the mixed lymphocyte culture test in the horse is described. The test is performed in either round- or flat-bottom microtitration tissue culture plates. Concentrations of responsing and stimulating cells are varied, depening on the experiment. Significant discrimination between isogeneic and allogenic mixtures is possible after 120 hours' culture when cells are labeled ([3H]thymidine) for the last 16 to 18 hours of the test.

Original languageEnglish (US)
Pages (from-to)337-339
Number of pages3
JournalAmerican Journal of Veterinary Research
Volume39
Issue number2
StatePublished - Feb 1 1978

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