TY - JOUR
T1 - MicroCT versus sTSLIM 3D Imaging of the mouse cochlea
AU - Buytaert, Jan A.N.
AU - Johnson, Shane B.
AU - Dierick, Manuel
AU - Salih, Wasil H.M.
AU - Santi, Peter A.
PY - 2013
Y1 - 2013
N2 - We made a qualitative and quantitative comparison between a state-of-the-art implementation of micro-Computed Tomography (microCT) and the scanning Thin-Sheet Laser Imaging Microscopy (sTSLIM) method, applied to mouse cochleae. Both imaging methods are non-destructive and perform optical sectioning, respectively, with X-rays and laser light. MicroCT can be used on fresh or fixed tissue samples and is primarily designed to image bone rather than soft tissues. It requires complex backprojection algorithms to produce a two-dimensional image, and it is an expensive instrument. sTSLIM requires that a specimen be chemically fixed, decalcified, and cleared; but it produces high-resolution images of soft and bony tissues with minimum image postprocessing and is less expensive than microCT. In this article, we discuss the merits and disadvantages of each method individually and when combined.
AB - We made a qualitative and quantitative comparison between a state-of-the-art implementation of micro-Computed Tomography (microCT) and the scanning Thin-Sheet Laser Imaging Microscopy (sTSLIM) method, applied to mouse cochleae. Both imaging methods are non-destructive and perform optical sectioning, respectively, with X-rays and laser light. MicroCT can be used on fresh or fixed tissue samples and is primarily designed to image bone rather than soft tissues. It requires complex backprojection algorithms to produce a two-dimensional image, and it is an expensive instrument. sTSLIM requires that a specimen be chemically fixed, decalcified, and cleared; but it produces high-resolution images of soft and bony tissues with minimum image postprocessing and is less expensive than microCT. In this article, we discuss the merits and disadvantages of each method individually and when combined.
KW - Light-sheet fluorescence microscopy
KW - Micro-computed tomography
KW - Mouse cochlea
KW - Orthogonal-plane fluorescence optical sectioning microscopy
KW - Scanning thin-sheet laser imaging microscopy
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U2 - 10.1369/0022155413478613
DO - 10.1369/0022155413478613
M3 - Article
C2 - 23360693
AN - SCOPUS:84879291620
SN - 0022-1554
VL - 61
SP - 382
EP - 395
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
IS - 5
ER -