@inproceedings{0b9dacbfa3574fe99821e612efb03b47,
title = "Microchip electrophoresis devices for the detection of nitric oxide: Comparison of bulk cell and single cell analysis",
abstract = "This paper describes the development of microchip electrophoresis (ME) devices for detection of nitric oxide (NO) from bulk cell lysates and single cells. Detection of NO and other endogenous electroactive species in Jurkat cells was achieved using ME with amperometric detection. Electrochemical (EC) results were then validated by ME coupled to laser-induced fluorescence (LIF) using 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FMDA), a NO-specific probe. In parallel, a single cell analysis device was developed for the determination of intracellular NO using DAF-FMDA and LIF detection.",
keywords = "Amperometric detection, Bulk cell analysis, Laser induced fluorescence detection, Microchip electrophoresis, Nitric oxide, Single cell analysis",
author = "Lunte, {Susan M.} and Gunasekara, {Dulan B.} and Metto, {Eve C.} and Hulvey, {Matthew K.} and Mainz, {Emilie R.} and Giuseppe Caruso and {Da Silva}, {Jose Alberto Fracassi} and Jensen, {Derek T.} and Culbertson, {Anne H.} and Grigsby, {Ryan J.} and Culbertson, {Christopher T.}",
year = "2011",
month = dec,
day = "1",
language = "English (US)",
isbn = "9781618395955",
series = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",
pages = "1728--1730",
booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",
note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",
}