Microbial hydrolysis and fermentation of rice straw for ethanol production

Xiaodan Wu, Jinsheng Zhang, Erni Xu, Yuhuan Liu, Yanling Cheng, Min Addy, Wenguang Zhou, Richard Griffith, Paul Chen, Roger Ruan

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


In this work, Trichoderma reesei Aq-5b and Trichoderma viride NSW-XM, capable of excreting lignin-degrading enzymes and cellulase were used to create a mixed culture system to hydrolyze rice straw in situ. The results showed that Aq-5b and NSW-XM were able to co-grow and produce complementary lignin degrading enzymes, namely laccase and lignin peroxidase. The two microorganisms were able to produce 22.74 g/L of reducing sugar in the optimal condition. For fermentation of the hydrolysates produced, Saccharomyces cerevisiae and Candida tropicalis NSW-NW were co-immobilized in polymer beads composed of sodium alginate, polyvinyl alcohol, and silicon dioxide (SA-PVA-SiO2). The beads remained intact after 4 cycles of use and provided satisfactory protection for the yeasts and a suitable microenvironment for ethanol fermentation, greatly improved the efficiency of ethanol production, shortened the fermentation cycle and achieved yeasts recycling for continuous or batch-fed fermentation. The productivity of ethanol reached up to 2.17 g/(L h).

Original languageEnglish (US)
Pages (from-to)679-686
Number of pages8
StatePublished - Sep 15 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd. All rights reserved.


  • Co-immobilization
  • Enzymatic hydrolysis in-situ
  • Lignin-degrading enzymes
  • Mixed culture
  • Rice straw


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