Microbial contamination of transplant solutions during pancreatic islet autotransplants is not associated with clinical infection in a pediatric population

Megan G. Berger, Kaustav Majumder, James S. Hodges, Melena D. Bellin, Sarah Jane Schwarzenberg, Sameer Gupta, Ty B. Dunn, Gregory J. Beilman, Timothy L. Pruett, Martin L. Freeman, Joshua J. Wilhelm, David E R Sutherland, Srinath Chinnakotla

Research output: Contribution to journalArticle

13 Scopus citations


Background/Objectives Total pancreatectomy and islet autotransplant (TP-IAT) is a potential treatment for children with severe refractory chronic pancreatitis. Cultures from the resected pancreas and final islet preparation are frequently positive for microbes. It is unknown whether positive cultures are associated with adverse outcomes in pediatric patients. Methods We reviewed the medical records of children (n = 86) who underwent TP-IAT from May 2006–March 2015 with emphasis on demographics, previous pancreatic interventions, culture results, islet yield, hospital days, posttransplant islet function, and posttransplant infections. We compared outcomes in patients with positive (n = 57) and negative (n = 29) cultures. Results Patients with positive cultures had higher rates of previous pancreas surgery (P = 0.007) and endoscopic retrograde cholangiopancreatography (P < 0.0001). Positive cultures were not associated with posttransplant infections (P = 1.00) or prolonged hospital length of stay (P = 0.29). Patients with positive final islet preparation culture showed increased rates of graft failure at 2 years posttransplant (P = 0.041), but not when adjusted for islet mass transplanted (P = 0.39). Conclusions Positive cultures during pediatric TP-IATs do not increase the risk of posttransplant infections or prolong hospital length of stay. Endocrine function depends on islet mass transplanted.

Original languageEnglish (US)
Pages (from-to)555-562
Number of pages8
Issue number4
StatePublished - Jul 1 2016



  • Chronic pancreatitis
  • Infection
  • Islet autotransplant
  • Pediatric
  • Sterility culture
  • Total pancreatectomy

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