Microbial communities in methane- and short chain alkane-rich hydrothermal sediments of Guaymas Basin

Frederick Dowell, Zena Cardman, Srishti Dasarathy, Matthias Y. Kellermann, Julius S. Lipp, S. Emil Ruff, Jennifer F. Biddle, Luke J. McKay, Barbara MacGregor, Karen G. Lloyd, Daniel B. Albert, Howard Mendlovitz, Kai Uwe Hinrichs, Andreas Teske

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and short chain alkane-oxidizing microbial communities. In this study, hot sediments (above 60°C) covered with sulfur-oxidizing microbial mats surrounding a hydrothermal mound (termed "Mat Mound") were characterized by porewater geochemistry of methane, C2-C6 short-chain alkanes, sulfate, sulfide, sulfate reduction rate measurements, in situ temperature gradients, bacterial and archaeal 16S rRNA gene clone libraries and V6 tag pyrosequencing. The most abundantly detected groups in the Mat mound sediments include anaerobic methane-oxidizing archaea of the ANME-1 lineage and its sister clade ANME-1Guaymas, the uncultured bacterial groups SEEP-SRB2 within the Deltaproteobacteria and the separately branching HotSeep-1 Group; these uncultured bacteria are candidates for sulfate-reducing alkane oxidation and for sulfate-reducing syntrophy with ANME archaea. The archaeal dataset indicates distinct habitat preferences for ANME-1, ANME-1-Guaymas, and ANME-2 archaea in Guaymas Basin hydrothermal sediments. The bacterial groups SEEP-SRB2 and HotSeep-1 co-occur with ANME-1 and ANME-1Guaymas in hydrothermally active sediments underneath microbial mats in Guaymas Basin. We propose the working hypothesis that this mixed bacterial and archaeal community catalyzes the oxidation of both methane and short-chain alkanes, and constitutes a microbial community signature that is characteristic for hydrothermal and/or cold seep sediments containing both substrates.

Original languageEnglish (US)
Article number00017
JournalFrontiers in Microbiology
Volume7
Issue numberJAN
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Alkanes
Methane
Sulfates
Archaea
Sulfides
Ecosystem
Deltaproteobacteria
Gulf of Mexico
Gene Library
rRNA Genes
Sulfur
Clone Cells
Bacteria
Temperature

Keywords

  • ANME
  • Alkanes
  • Guaymas Basin
  • Hydrothermal vents
  • Methane
  • Sediment

Cite this

Dowell, F., Cardman, Z., Dasarathy, S., Kellermann, M. Y., Lipp, J. S., Ruff, S. E., ... Teske, A. (2016). Microbial communities in methane- and short chain alkane-rich hydrothermal sediments of Guaymas Basin. Frontiers in Microbiology, 7(JAN), [00017]. https://doi.org/10.3389/fmicb.2016.00017

Microbial communities in methane- and short chain alkane-rich hydrothermal sediments of Guaymas Basin. / Dowell, Frederick; Cardman, Zena; Dasarathy, Srishti; Kellermann, Matthias Y.; Lipp, Julius S.; Ruff, S. Emil; Biddle, Jennifer F.; McKay, Luke J.; MacGregor, Barbara; Lloyd, Karen G.; Albert, Daniel B.; Mendlovitz, Howard; Hinrichs, Kai Uwe; Teske, Andreas.

In: Frontiers in Microbiology, Vol. 7, No. JAN, 00017, 01.01.2016.

Research output: Contribution to journalArticle

Dowell, F, Cardman, Z, Dasarathy, S, Kellermann, MY, Lipp, JS, Ruff, SE, Biddle, JF, McKay, LJ, MacGregor, B, Lloyd, KG, Albert, DB, Mendlovitz, H, Hinrichs, KU & Teske, A 2016, 'Microbial communities in methane- and short chain alkane-rich hydrothermal sediments of Guaymas Basin', Frontiers in Microbiology, vol. 7, no. JAN, 00017. https://doi.org/10.3389/fmicb.2016.00017
Dowell, Frederick ; Cardman, Zena ; Dasarathy, Srishti ; Kellermann, Matthias Y. ; Lipp, Julius S. ; Ruff, S. Emil ; Biddle, Jennifer F. ; McKay, Luke J. ; MacGregor, Barbara ; Lloyd, Karen G. ; Albert, Daniel B. ; Mendlovitz, Howard ; Hinrichs, Kai Uwe ; Teske, Andreas. / Microbial communities in methane- and short chain alkane-rich hydrothermal sediments of Guaymas Basin. In: Frontiers in Microbiology. 2016 ; Vol. 7, No. JAN.
@article{75fcae39af9746e79dcbbba6f50cfb17,
title = "Microbial communities in methane- and short chain alkane-rich hydrothermal sediments of Guaymas Basin",
abstract = "The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and short chain alkane-oxidizing microbial communities. In this study, hot sediments (above 60°C) covered with sulfur-oxidizing microbial mats surrounding a hydrothermal mound (termed {"}Mat Mound{"}) were characterized by porewater geochemistry of methane, C2-C6 short-chain alkanes, sulfate, sulfide, sulfate reduction rate measurements, in situ temperature gradients, bacterial and archaeal 16S rRNA gene clone libraries and V6 tag pyrosequencing. The most abundantly detected groups in the Mat mound sediments include anaerobic methane-oxidizing archaea of the ANME-1 lineage and its sister clade ANME-1Guaymas, the uncultured bacterial groups SEEP-SRB2 within the Deltaproteobacteria and the separately branching HotSeep-1 Group; these uncultured bacteria are candidates for sulfate-reducing alkane oxidation and for sulfate-reducing syntrophy with ANME archaea. The archaeal dataset indicates distinct habitat preferences for ANME-1, ANME-1-Guaymas, and ANME-2 archaea in Guaymas Basin hydrothermal sediments. The bacterial groups SEEP-SRB2 and HotSeep-1 co-occur with ANME-1 and ANME-1Guaymas in hydrothermally active sediments underneath microbial mats in Guaymas Basin. We propose the working hypothesis that this mixed bacterial and archaeal community catalyzes the oxidation of both methane and short-chain alkanes, and constitutes a microbial community signature that is characteristic for hydrothermal and/or cold seep sediments containing both substrates.",
keywords = "ANME, Alkanes, Guaymas Basin, Hydrothermal vents, Methane, Sediment",
author = "Frederick Dowell and Zena Cardman and Srishti Dasarathy and Kellermann, {Matthias Y.} and Lipp, {Julius S.} and Ruff, {S. Emil} and Biddle, {Jennifer F.} and McKay, {Luke J.} and Barbara MacGregor and Lloyd, {Karen G.} and Albert, {Daniel B.} and Howard Mendlovitz and Hinrichs, {Kai Uwe} and Andreas Teske",
year = "2016",
month = "1",
day = "1",
doi = "10.3389/fmicb.2016.00017",
language = "English (US)",
volume = "7",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
number = "JAN",

}

TY - JOUR

T1 - Microbial communities in methane- and short chain alkane-rich hydrothermal sediments of Guaymas Basin

AU - Dowell, Frederick

AU - Cardman, Zena

AU - Dasarathy, Srishti

AU - Kellermann, Matthias Y.

AU - Lipp, Julius S.

AU - Ruff, S. Emil

AU - Biddle, Jennifer F.

AU - McKay, Luke J.

AU - MacGregor, Barbara

AU - Lloyd, Karen G.

AU - Albert, Daniel B.

AU - Mendlovitz, Howard

AU - Hinrichs, Kai Uwe

AU - Teske, Andreas

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and short chain alkane-oxidizing microbial communities. In this study, hot sediments (above 60°C) covered with sulfur-oxidizing microbial mats surrounding a hydrothermal mound (termed "Mat Mound") were characterized by porewater geochemistry of methane, C2-C6 short-chain alkanes, sulfate, sulfide, sulfate reduction rate measurements, in situ temperature gradients, bacterial and archaeal 16S rRNA gene clone libraries and V6 tag pyrosequencing. The most abundantly detected groups in the Mat mound sediments include anaerobic methane-oxidizing archaea of the ANME-1 lineage and its sister clade ANME-1Guaymas, the uncultured bacterial groups SEEP-SRB2 within the Deltaproteobacteria and the separately branching HotSeep-1 Group; these uncultured bacteria are candidates for sulfate-reducing alkane oxidation and for sulfate-reducing syntrophy with ANME archaea. The archaeal dataset indicates distinct habitat preferences for ANME-1, ANME-1-Guaymas, and ANME-2 archaea in Guaymas Basin hydrothermal sediments. The bacterial groups SEEP-SRB2 and HotSeep-1 co-occur with ANME-1 and ANME-1Guaymas in hydrothermally active sediments underneath microbial mats in Guaymas Basin. We propose the working hypothesis that this mixed bacterial and archaeal community catalyzes the oxidation of both methane and short-chain alkanes, and constitutes a microbial community signature that is characteristic for hydrothermal and/or cold seep sediments containing both substrates.

AB - The hydrothermal sediments of Guaymas Basin, an active spreading center in the Gulf of California (Mexico), are rich in porewater methane, short-chain alkanes, sulfate and sulfide, and provide a model system to explore habitat preferences of microorganisms, including sulfate-dependent, methane- and short chain alkane-oxidizing microbial communities. In this study, hot sediments (above 60°C) covered with sulfur-oxidizing microbial mats surrounding a hydrothermal mound (termed "Mat Mound") were characterized by porewater geochemistry of methane, C2-C6 short-chain alkanes, sulfate, sulfide, sulfate reduction rate measurements, in situ temperature gradients, bacterial and archaeal 16S rRNA gene clone libraries and V6 tag pyrosequencing. The most abundantly detected groups in the Mat mound sediments include anaerobic methane-oxidizing archaea of the ANME-1 lineage and its sister clade ANME-1Guaymas, the uncultured bacterial groups SEEP-SRB2 within the Deltaproteobacteria and the separately branching HotSeep-1 Group; these uncultured bacteria are candidates for sulfate-reducing alkane oxidation and for sulfate-reducing syntrophy with ANME archaea. The archaeal dataset indicates distinct habitat preferences for ANME-1, ANME-1-Guaymas, and ANME-2 archaea in Guaymas Basin hydrothermal sediments. The bacterial groups SEEP-SRB2 and HotSeep-1 co-occur with ANME-1 and ANME-1Guaymas in hydrothermally active sediments underneath microbial mats in Guaymas Basin. We propose the working hypothesis that this mixed bacterial and archaeal community catalyzes the oxidation of both methane and short-chain alkanes, and constitutes a microbial community signature that is characteristic for hydrothermal and/or cold seep sediments containing both substrates.

KW - ANME

KW - Alkanes

KW - Guaymas Basin

KW - Hydrothermal vents

KW - Methane

KW - Sediment

UR - http://www.scopus.com/inward/record.url?scp=84957989787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957989787&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2016.00017

DO - 10.3389/fmicb.2016.00017

M3 - Article

VL - 7

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - JAN

M1 - 00017

ER -