Microbial communities associated with phosphogenic sediments and phosphoclast-associated DNA of the Benguela upwelling system

Roman Zoss, Fernando Medina Ferrer, Beverly E Flood, Daniel S Jones, Deon C. Louw, Jake Bailey

Research output: Contribution to journalArticle

Abstract

The processes that lead to the precipitation of authigenic calcium phosphate minerals in certain marine pore waters remain poorly understood. Phosphogenesis occurs in sediments beneath some oceanic upwelling zones that harbor polyphosphate-accumulating bacteria. These bacteria are believed to concentrate phosphate in sediment pore waters, creating supersaturated conditions with respect to apatite precursors. However, the relationship between microbes and phosphorite formation is not fully resolved. To further study this association, we examined microbial community data generated from two sources: sediment cores recovered from the shelf of the Benguela upwelling region where phosphorites are currently forming, and DNA preserved within phosphoclasts recovered from a phosphorite deposit along the Benguela shelf. iTag and clone library sequencing of the 16S rRNA gene showed that many of our sediment-hosted communities shared large numbers of phylotypes with one another, and that the same metabolic guilds were represented at localities across the shelf. Sulfate-reducing bacteria and sulfur-oxidizing bacteria were particularly abundant in our datasets, as were phylotypes that are known to carry out nitrification and the anaerobic oxidation of ammonium. The DNA extracted from phosphoclasts contained the signature of a distinct microbial community from those observed in the modern sediments. While some aspects of the modern and phosphoclast communities were similar, we observed both an enrichment of certain common microbial classes found in the modern phosphogenic sediments and a relative depletion of others. The phosphoclast-associated DNA could represent a relict signature of one or more microbial assemblages that were present when the apatite or its precursors precipitated. While these taxa may or may not have contributed to the precipitation of the apatite that now hosts their genetic remains, several groups represented in the phosphoclast extract dataset have the genetic potential to metabolize polyphosphate, and perhaps modulate phosphate concentrations in pore waters where carbonate fluorapatite (or its precursors) are known to be precipitating.

Original languageEnglish (US)
Pages (from-to)76-90
Number of pages15
JournalGeobiology
Volume17
Issue number1
DOIs
StatePublished - Jan 1 2019

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microbial communities
microbial community
apatite
upwelling
phosphorite
DNA
sediments
porewater
sediment
phosphate
polyphosphates
bacterium
phosphogenesis
bacteria
fluorapatite
phosphate minerals
phosphates
sulfate-reducing bacterium
sulfate-reducing bacteria
guild

Keywords

  • phosphorite
  • polyphosphate
  • sulfur bacteria
  • upwelling

Cite this

Microbial communities associated with phosphogenic sediments and phosphoclast-associated DNA of the Benguela upwelling system. / Zoss, Roman; Medina Ferrer, Fernando; Flood, Beverly E; Jones, Daniel S; Louw, Deon C.; Bailey, Jake.

In: Geobiology, Vol. 17, No. 1, 01.01.2019, p. 76-90.

Research output: Contribution to journalArticle

Zoss, Roman ; Medina Ferrer, Fernando ; Flood, Beverly E ; Jones, Daniel S ; Louw, Deon C. ; Bailey, Jake. / Microbial communities associated with phosphogenic sediments and phosphoclast-associated DNA of the Benguela upwelling system. In: Geobiology. 2019 ; Vol. 17, No. 1. pp. 76-90.
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abstract = "The processes that lead to the precipitation of authigenic calcium phosphate minerals in certain marine pore waters remain poorly understood. Phosphogenesis occurs in sediments beneath some oceanic upwelling zones that harbor polyphosphate-accumulating bacteria. These bacteria are believed to concentrate phosphate in sediment pore waters, creating supersaturated conditions with respect to apatite precursors. However, the relationship between microbes and phosphorite formation is not fully resolved. To further study this association, we examined microbial community data generated from two sources: sediment cores recovered from the shelf of the Benguela upwelling region where phosphorites are currently forming, and DNA preserved within phosphoclasts recovered from a phosphorite deposit along the Benguela shelf. iTag and clone library sequencing of the 16S rRNA gene showed that many of our sediment-hosted communities shared large numbers of phylotypes with one another, and that the same metabolic guilds were represented at localities across the shelf. Sulfate-reducing bacteria and sulfur-oxidizing bacteria were particularly abundant in our datasets, as were phylotypes that are known to carry out nitrification and the anaerobic oxidation of ammonium. The DNA extracted from phosphoclasts contained the signature of a distinct microbial community from those observed in the modern sediments. While some aspects of the modern and phosphoclast communities were similar, we observed both an enrichment of certain common microbial classes found in the modern phosphogenic sediments and a relative depletion of others. The phosphoclast-associated DNA could represent a relict signature of one or more microbial assemblages that were present when the apatite or its precursors precipitated. While these taxa may or may not have contributed to the precipitation of the apatite that now hosts their genetic remains, several groups represented in the phosphoclast extract dataset have the genetic potential to metabolize polyphosphate, and perhaps modulate phosphate concentrations in pore waters where carbonate fluorapatite (or its precursors) are known to be precipitating.",
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