Microbial and Reactive Transport Modeling Evidence for Hyporheic Flux-Driven Cryptic Sulfur Cycling and Anaerobic Methane Oxidation in a Sulfate-Impacted Wetland-Stream System

Gene Hua Crystal Ng, Carla E. Rosenfeld, Cara M. Santelli, Amanda R. Yourd, Jack Lange, Kelly Duhn, Nathan W. Johnson

Research output: Contribution to journalArticlepeer-review

Abstract

This study reexamines the common expectations that in freshwater systems, sulfur plays a minor role in carbon cycling, and aerobic processes dominate methane oxidation. In anoxic sediments of a sulfate-impacted wetland-stream system in Minnesota (USA), a reactive transport model calibrated to geochemical observations predicted sulfate reduction to be the major terminal electron accepting process, and it showed that anaerobic oxidation of methane predominantly coupled with sulfate reduction attenuated methane concentrations near the sediment-water interface. Consistent with model results, 16S rRNA microbiome analysis revealed a high relative abundance of taxa capable of dissimilatory sulfate reduction. It further supported the conclusion that high simulated sulfate reduction rates could be maintained by a “cryptic” sulfur cycle coupled to iron and methane. Low relative abundance of known iron reducing bacteria raised the possibility of abiotic ferric iron (Fe) reduction driving sulfide reoxidation to intermediate-valence sulfur forms; widespread potential for microbially mediated disproportionation, oxidation, and reduction of sulfur intermediates provided mechanisms for completing redox cycles; and archaea comprising up to 25% of the microbial community could include consortia capable of anaerobic oxidation of methane. These biogeochemical processes were found to be controlled by hyporheic fluxes. Lower-magnitude fluxes in wetland compared to channel sediments created sharper geochemical gradients that generated greater heterogeneity in microbial distributions and reaction rates. Changes in upward flux caused fluctuations in sulfate concentrations that led to alternating simulations of methane production and transport. Our work supports the importance of hyporheic flux-driven iron-sulfur-methane cycling in sulfate-impacted wetlands and prompts further investigations under freshwater conditions.

Original languageEnglish (US)
Article numbere2019JG005185
JournalJournal of Geophysical Research: Biogeosciences
Volume125
Issue number2
DOIs
StatePublished - Feb 1 2020

Bibliographical note

Funding Information:
To support this work, G.-H.?C. Ng received funds from the Department of Earth Sciences, and C. Santelli received funds from MnDRIVE Environment, both at the University of Minnesota, Twin Cities. The authors would like to acknowledge Patrick O'Hara (University of Minnesota-Twin Cities) for graphical assistance in creating Figures and. Joshua Torgeson and Liz Roepke from University of Minnesota, Twin Cities, and Daniel Fraser and Sophie LaFond from University of Minnesota, Duluth, provided field assistance. Chad Sandell (University of Minnesota, Twin Cities) helped design and construct temperature probes for determining hyporheic flux. We thank two anonymous reviewers and the associate editor for their helpful comments. Hydrologic and geochemical data are publically available in the EDI (Environmental Data Initiative) Data Repository (https://doi.org/10.6073/pasta/611c867faf4da200141246c8e9c494c5). Microbial metadata and raw DNA sequences are publically available in the NCBI Sequence Read Archive under BioProject PRJNA530072 and Biosamples SAMN11292835 to SAMN11292879.

Keywords

  • 16S rRNA microbiome analysis
  • anaerobic methane oxidation
  • cryptic sulfur cycling
  • hyporheic zone
  • reactive transport modeling
  • wetlands

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