Microalgal swimming signatures and neutral lipids production across growth phases

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3 Scopus citations

Abstract

Microalgae have been shown as a potential bioresource for food, biofuel, and pharmaceutical products. During the growth phases with corresponding environmental conditions, microalgae accumulate different amounts of various metabolites. We quantified the neutral lipids accumulation and analyzed the swimming signatures (speed and trajectories) of the motile green alga, Dunaliella primolecta, during the lag–exponential–stationary growth cycle at different nutrient concentrations. We discovered significant changes in the neutral lipid content and swimming signatures of microalgae across growth phases. The timing of the maximum swimming speed coincided with the maximum neutral lipid content and both maxima occurred under nutrient stress at the stationary growth phase. Furthermore, the swimming trajectories suggested statistically significant changes in swimming modes at the stationary growth phase when the maximum intracellular neutral lipid content was observed. Our results provide the potential exploitation of microalgal swimming signatures as possible indicators of the cultivation conditions and the timing of microalgal harvest to maximize the lipid yield for biofuel production. The findings can also be implemented to explore the production of food and antibiotics from other microalgal metabolites with low energy costs.

Original languageEnglish (US)
Pages (from-to)970-980
Number of pages11
JournalBiotechnology and bioengineering
Volume117
Issue number4
DOIs
StatePublished - Apr 1 2020

Bibliographical note

Funding Information:
The work was supported by the Legislative‐Citizen Commission on Minnesota Resources, Environment and Natural Resources Trust Fund 2015–2016 (Assessing the Increasing Harmful Algal Blooms in Minnesota Lakes, ID:038‐B); and the National Science Foundation National Robotics Initiative award (NSF‐IIS‐1427014). The cell imaging was done using the Nikon Eclipse 90i microscope with the assistance of Dr. Guillermo Marques and John Oja at the University of Minnesota—University Imaging Centers. The authors would also like to thank Dr. Wenqi Cui and Enxiang Zhang for the technical support in neutral lipid determination, Dr. Dan Troolin for technical support in micro‐PTV measurements and Jaewoo Jeong for part of the holography image processing.

Funding Information:
The work was supported by the Legislative-Citizen Commission on Minnesota Resources, Environment and Natural Resources Trust Fund 2015?2016 (Assessing the Increasing Harmful Algal Blooms in Minnesota Lakes, ID:038-B); and the National Science Foundation National Robotics Initiative award (NSF-IIS-1427014). The cell imaging was done using the Nikon Eclipse 90i microscope with the assistance of Dr. Guillermo Marques and John Oja at the University of Minnesota?University Imaging Centers. The authors would also like to thank Dr. Wenqi Cui and Enxiang Zhang for the technical support in neutral lipid determination, Dr. Dan Troolin for technical support in micro-PTV measurements and Jaewoo Jeong for part of the holography image processing.

Publisher Copyright:
© 2020 Wiley Periodicals, Inc.

Keywords

  • biofuel
  • lipids
  • microalgae
  • swimming

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.

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