Micro free flow electrophoresis

Alexander C. Johnson, Michael T. Bowser

Research output: Contribution to journalReview articlepeer-review

40 Scopus citations


Micro free-flow electrophoresis (μFFE) is a continuous separation technique in which analytes are streamed through a perpendicularly applied electric field in a planar separation channel. Analyte streams are deflected laterally based on their electrophoretic mobilities as they flow through the separation channel. A number of μFFE separation modes have been demonstrated, including free zone (FZ), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and isotachophoresis (ITP). Approximately 60 articles have been published since the first μFFE device was fabricated in 1994. We anticipate that recent advances in device design, detection, and fabrication, will allow μFFE to be applied to a much wider range of applications. Applications particularly well suited for μFFE analysis include continuous, real time monitoring and microscale purifications.

Original languageEnglish (US)
Pages (from-to)27-40
Number of pages14
JournalLab on a chip
Issue number1
StatePublished - Jan 7 2018

Bibliographical note

Funding Information:
The authors gratefully acknowledge funding from the National Science Foundation (CHE-1152022) and the National Institutes of Health (GM063533).

Publisher Copyright:
© 2018 The Royal Society of Chemistry.


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