TY - JOUR
T1 - Micro free flow electrophoresis
AU - Johnson, Alexander C.
AU - Bowser, Michael T.
N1 - Funding Information:
The authors gratefully acknowledge funding from the National Science Foundation (CHE-1152022) and the National Institutes of Health (GM063533).
PY - 2018/1/7
Y1 - 2018/1/7
N2 - Micro free-flow electrophoresis (μFFE) is a continuous separation technique in which analytes are streamed through a perpendicularly applied electric field in a planar separation channel. Analyte streams are deflected laterally based on their electrophoretic mobilities as they flow through the separation channel. A number of μFFE separation modes have been demonstrated, including free zone (FZ), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and isotachophoresis (ITP). Approximately 60 articles have been published since the first μFFE device was fabricated in 1994. We anticipate that recent advances in device design, detection, and fabrication, will allow μFFE to be applied to a much wider range of applications. Applications particularly well suited for μFFE analysis include continuous, real time monitoring and microscale purifications.
AB - Micro free-flow electrophoresis (μFFE) is a continuous separation technique in which analytes are streamed through a perpendicularly applied electric field in a planar separation channel. Analyte streams are deflected laterally based on their electrophoretic mobilities as they flow through the separation channel. A number of μFFE separation modes have been demonstrated, including free zone (FZ), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and isotachophoresis (ITP). Approximately 60 articles have been published since the first μFFE device was fabricated in 1994. We anticipate that recent advances in device design, detection, and fabrication, will allow μFFE to be applied to a much wider range of applications. Applications particularly well suited for μFFE analysis include continuous, real time monitoring and microscale purifications.
UR - http://www.scopus.com/inward/record.url?scp=85038631527&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85038631527&partnerID=8YFLogxK
U2 - 10.1039/c7lc01105a
DO - 10.1039/c7lc01105a
M3 - Review article
C2 - 29077103
AN - SCOPUS:85038631527
VL - 18
SP - 27
EP - 40
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 1
ER -