Micellar electrokinetic capillary chromatography reveals differences in intracellular metabolism between liposomal and free doxorubicin treatment of human leukemia cells

Angela R. Eder, Edgar A. Arriaga

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Doxil® is a pegylated liposome formulation of the anthracycline doxorubicin. To better explain observed differences in the toxicity of Doxil® and free doxorubicin in solution, the intracellular metabolism of the formulations after treatment in CCRF-CEM and CEM/C2 human leukemia cell lines was investigated. Using micellar electrokinetic capillary chromatography with laser-induced fluorescence detection, with a 63 zepto (10-21) mole doxorubicin limit of detection, five common metabolites and doxorubicin were detected upon treatment with both of these drug delivery systems. Two unique metabolites appeared with the Doxil® and two unique metabolites appeared with the free doxorubicin delivery systems. For common metabolites, the relative amount of metabolite generated from Doxil® was approximately 10 times higher than for free doxorubicin.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume829
Issue number1-2
DOIs
StatePublished - Dec 27 2005

Keywords

  • Capillary
  • Doxil
  • Doxorubicin
  • Electrophoresis
  • Laser-induced fluorescence
  • Liposome
  • Metabolism
  • Micellar electrokinetic capillary chromatography

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