Mice deficient for a small cluster of Piwi-lnteracting RNAs Implicate Piwi-interacting RNAs in transposon control

Mingang Xu, Yun You, Patricia Hunsicker, Tamaki Hori, Chris Small, Michael D. Griswold, Norman B. Hecht

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The mammalian testis expresses a class of small noncoding RNAs that interact with mammalian PIWI proteins. In mice, the PIWI-interacting RNAs (piRNAs) partner with mammalian PIWI proteins, PIWIL1 and PIWIL2, also known as MIWI and MILI, to maintain transposon silencing in the germline genome. Here, we demonstrate that inactivation of Nct1/2, two noncoding RNAs encoding piRNAs, leads to derepression of LINE-1 (L1) but does not affect mouse viability, spermatogenesis, testicular gene expression, or fertility. These findings indicate that piRNAs from a cluster on chromosome 2 are necessary to maintain transposon silencing.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalBiology of reproduction
Volume79
Issue number1
DOIs
StatePublished - Jul 2008

Keywords

  • Gametogenesis
  • Gene regulation
  • Male reproductive tract
  • Spermatogenesis
  • Testis
  • Transposon
  • piRNA

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