Methods for reactive oxygen species (ROS) detection in aqueous environments

Justina M. Burns, William J. Cooper, John L. Ferry, D. Whitney King, Brian P. DiMento, Kristopher McNeill, Christopher J. Miller, William L. Miller, Barrie M. Peake, Steven A. Rusak, Andrew L. Rose, T. David Waite

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321 Scopus citations


This review summarizes direct and indirect analytical methods for the detection and quantification of the reactive oxygen species (ROS): 1O2, O2·-/HOO·, H2O2, HO·, and CO3·- in aqueous solution. Each section briefly describes the chemical properties of a specific ROS followed by a table (organized alphabetically by detection method, i. e., absorbance, chemiluminescence, etc.) summarizing the nature of the observable (associated analytical signal) for each method, limit of detection, application notes, and reaction of the probe molecule with the particular ROS.

Original languageEnglish (US)
Pages (from-to)683-734
Number of pages52
JournalAquatic Sciences
Issue number4
StatePublished - Oct 2012

Bibliographical note

Funding Information:
Acknowledgments The authors would like to acknowledge valuable comments of the anonymous reviewers and the associate editor which significantly improved the manuscript. This work was supported in part by the US National Science Foundation, Grant no. OCE 0752473 and the College of Arts and Sciences at the University of South Carolina. This is publication 72 of the University of California Irvine, Urban Water Research Center.


  • Analysis
  • Aquatic environment
  • Carbonate
  • Hydrogen peroxide
  • Hydroxyl
  • Radical
  • Reactive oxygen species
  • Singlet oxygen
  • Superoxide
  • Transient


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