Methodologic advancements in the study of airway smooth muscle

Michael I. Kotlikoff; Mathur S. Kannan; Julian Solway; Ke Yu Deng; Deepak A. Deshpande; Maria Dowell; Morris Feldman; Kai Su Green; Guangju Ji; Robyn Johnston; Oren Lakser; Jane Lee; Frances E. Lund; Carlos Milla; Richard W. Mitchell; Junichi Nakai; Mark Rishniw; Timothy F. Walseth; Thomas A. White; Jason Wilson; Hong Bo Xin; Prescott G. Woodruff

doi:10.1016/j.jaci.2004.04.040

Methodologic advancements in the study of airway smooth muscle

Michael I. Kotlikoff, Mathur S. Kannan, Julian Solway, Ke Yu Deng, Deepak A. Deshpande, Maria Dowell, Morris Feldman, Kai Su Green, Guangju Ji, Robyn Johnston, Oren Lakser, Jane Lee, Frances E. Lund, Carlos Milla, Richard W. Mitchell, Junichi Nakai, Mark Rishniw, Timothy F. Walseth, Thomas A. White, Jason WilsonHong Bo Xin, Prescott G. Woodruff

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The study of isolated airway myocytes has provided important information relative to specific processes that regulate contraction, proliferation, and synthetic properties of airway smooth muscle (ASM). To place this information in physiological context, however, improved methods to examine airway biology in vivo are needed. Advances in genetic, biochemical, and optical methods provide unprecedented opportunities to improve our understanding of in vivo physiology and pathophysiology. This article describes 4 important methodologic advances in the study of ASM: (1) the development of transgenic mice that could be used to investigate ASM proliferation and phenotype switching during the development of hypersensitivity, and to investigate excitation-contraction coupling; (2) the use of CD38-deficient mice to confirm the role of CD38-dependent, cyclic adenosine diphosphate-ribose-mediated calcium release in airway responsiveness; (3) investigation of the role of actin filament length and p38 mitogen-activated protein kinase activity in regulating the mechanical plasticity-elasticity balance in contracted ASM; and (d) the use of bronchial biopsies to study ASM structure and phenotype in respiratory science.

Original language	English (US)
Pages (from-to)	S18-S31
Journal	Journal of Allergy and Clinical Immunology
Volume	114
Issue number	2 SUPPL.
DOIs	https://doi.org/10.1016/j.jaci.2004.04.040
State	Published - Aug 2004

Bibliographical note

Funding Information:
Supported by grants HL45239, DK58795, and DK065992 from the National Institutes of Health (Dr Kotlikoff), grants from the Academic Health Center, University of Minnesota (Dr Walseth and Dr Kannan), grants HL56399, AI56352, and HL07605 from the National Institutes of Health (Dr Solway), and support by K23 RR17002 and the General Clinical Research Center, Moffitt Hospital, University of California, San Francisco, with funds provided by the National Center for Research Resources, 5 M01 RR-00079, US Public Health Service (Dr Woodruff).

Keywords

ASM
Airway smooth muscle
Asthma
CD38/cyclic ADP-ribose pathway
airway smooth muscle
bronchial biopsy
cADPR
calcium homeostasis
excitation-contraction coupling
morphometric changes
phenotype
plasticity- elasticity balance
proliferation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jaci.2004.04.040

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Kotlikoff, M. I., Kannan, M. S., Solway, J., Deng, K. Y., Deshpande, D. A., Dowell, M., Feldman, M., Green, K. S., Ji, G., Johnston, R., Lakser, O., Lee, J., Lund, F. E., Milla, C., Mitchell, R. W., Nakai, J., Rishniw, M., Walseth, T. F., White, T. A., ... Woodruff, P. G. (2004). Methodologic advancements in the study of airway smooth muscle. Journal of Allergy and Clinical Immunology, 114(2 SUPPL.), S18-S31. https://doi.org/10.1016/j.jaci.2004.04.040

Kotlikoff, MI, Kannan, MS, Solway, J, Deng, KY, Deshpande, DA, Dowell, M, Feldman, M, Green, KS, Ji, G, Johnston, R, Lakser, O, Lee, J, Lund, FE, Milla, C, Mitchell, RW, Nakai, J, Rishniw, M, Walseth, TF, White, TA, Wilson, J, Xin, HB & Woodruff, PG 2004, 'Methodologic advancements in the study of airway smooth muscle', Journal of Allergy and Clinical Immunology, vol. 114, no. 2 SUPPL., pp. S18-S31. https://doi.org/10.1016/j.jaci.2004.04.040

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abstract = "The study of isolated airway myocytes has provided important information relative to specific processes that regulate contraction, proliferation, and synthetic properties of airway smooth muscle (ASM). To place this information in physiological context, however, improved methods to examine airway biology in vivo are needed. Advances in genetic, biochemical, and optical methods provide unprecedented opportunities to improve our understanding of in vivo physiology and pathophysiology. This article describes 4 important methodologic advances in the study of ASM: (1) the development of transgenic mice that could be used to investigate ASM proliferation and phenotype switching during the development of hypersensitivity, and to investigate excitation-contraction coupling; (2) the use of CD38-deficient mice to confirm the role of CD38-dependent, cyclic adenosine diphosphate-ribose-mediated calcium release in airway responsiveness; (3) investigation of the role of actin filament length and p38 mitogen-activated protein kinase activity in regulating the mechanical plasticity-elasticity balance in contracted ASM; and (d) the use of bronchial biopsies to study ASM structure and phenotype in respiratory science.",

keywords = "ASM, Airway smooth muscle, Asthma, CD38/cyclic ADP-ribose pathway, airway smooth muscle, bronchial biopsy, cADPR, calcium homeostasis, excitation-contraction coupling, morphometric changes, phenotype, plasticity- elasticity balance, proliferation",

author = "Kotlikoff, {Michael I.} and Kannan, {Mathur S.} and Julian Solway and Deng, {Ke Yu} and Deshpande, {Deepak A.} and Maria Dowell and Morris Feldman and Green, {Kai Su} and Guangju Ji and Robyn Johnston and Oren Lakser and Jane Lee and Lund, {Frances E.} and Carlos Milla and Mitchell, {Richard W.} and Junichi Nakai and Mark Rishniw and Walseth, {Timothy F.} and White, {Thomas A.} and Jason Wilson and Xin, {Hong Bo} and Woodruff, {Prescott G.}",

note = "Funding Information: Supported by grants HL45239, DK58795, and DK065992 from the National Institutes of Health (Dr Kotlikoff), grants from the Academic Health Center, University of Minnesota (Dr Walseth and Dr Kannan), grants HL56399, AI56352, and HL07605 from the National Institutes of Health (Dr Solway), and support by K23 RR17002 and the General Clinical Research Center, Moffitt Hospital, University of California, San Francisco, with funds provided by the National Center for Research Resources, 5 M01 RR-00079, US Public Health Service (Dr Woodruff). ",

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AU - Kotlikoff, Michael I.

AU - Kannan, Mathur S.

AU - Solway, Julian

AU - Deng, Ke Yu

AU - Deshpande, Deepak A.

AU - Dowell, Maria

AU - Feldman, Morris

AU - Green, Kai Su

AU - Ji, Guangju

AU - Johnston, Robyn

AU - Lakser, Oren

AU - Lee, Jane

AU - Lund, Frances E.

AU - Milla, Carlos

AU - Mitchell, Richard W.

AU - Nakai, Junichi

AU - Rishniw, Mark

AU - Walseth, Timothy F.

AU - White, Thomas A.

AU - Wilson, Jason

AU - Xin, Hong Bo

AU - Woodruff, Prescott G.

N1 - Funding Information: Supported by grants HL45239, DK58795, and DK065992 from the National Institutes of Health (Dr Kotlikoff), grants from the Academic Health Center, University of Minnesota (Dr Walseth and Dr Kannan), grants HL56399, AI56352, and HL07605 from the National Institutes of Health (Dr Solway), and support by K23 RR17002 and the General Clinical Research Center, Moffitt Hospital, University of California, San Francisco, with funds provided by the National Center for Research Resources, 5 M01 RR-00079, US Public Health Service (Dr Woodruff).

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N2 - The study of isolated airway myocytes has provided important information relative to specific processes that regulate contraction, proliferation, and synthetic properties of airway smooth muscle (ASM). To place this information in physiological context, however, improved methods to examine airway biology in vivo are needed. Advances in genetic, biochemical, and optical methods provide unprecedented opportunities to improve our understanding of in vivo physiology and pathophysiology. This article describes 4 important methodologic advances in the study of ASM: (1) the development of transgenic mice that could be used to investigate ASM proliferation and phenotype switching during the development of hypersensitivity, and to investigate excitation-contraction coupling; (2) the use of CD38-deficient mice to confirm the role of CD38-dependent, cyclic adenosine diphosphate-ribose-mediated calcium release in airway responsiveness; (3) investigation of the role of actin filament length and p38 mitogen-activated protein kinase activity in regulating the mechanical plasticity-elasticity balance in contracted ASM; and (d) the use of bronchial biopsies to study ASM structure and phenotype in respiratory science.

AB - The study of isolated airway myocytes has provided important information relative to specific processes that regulate contraction, proliferation, and synthetic properties of airway smooth muscle (ASM). To place this information in physiological context, however, improved methods to examine airway biology in vivo are needed. Advances in genetic, biochemical, and optical methods provide unprecedented opportunities to improve our understanding of in vivo physiology and pathophysiology. This article describes 4 important methodologic advances in the study of ASM: (1) the development of transgenic mice that could be used to investigate ASM proliferation and phenotype switching during the development of hypersensitivity, and to investigate excitation-contraction coupling; (2) the use of CD38-deficient mice to confirm the role of CD38-dependent, cyclic adenosine diphosphate-ribose-mediated calcium release in airway responsiveness; (3) investigation of the role of actin filament length and p38 mitogen-activated protein kinase activity in regulating the mechanical plasticity-elasticity balance in contracted ASM; and (d) the use of bronchial biopsies to study ASM structure and phenotype in respiratory science.

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KW - Airway smooth muscle

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KW - airway smooth muscle

KW - bronchial biopsy

KW - cADPR

KW - calcium homeostasis

KW - excitation-contraction coupling

KW - morphometric changes

KW - phenotype

KW - plasticity- elasticity balance

KW - proliferation

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SP - S18-S31

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IS - 2 SUPPL.

ER -

Methodologic advancements in the study of airway smooth muscle

Abstract

Bibliographical note

Keywords

UN SDGs

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