Method for the concentration of infectious pancreatic necrosis virus from hatchery water

S. Maheshkumar, Sagar M. Goyal, Richard B. Peterson, Philip P. Economon

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A method was developed for concentrating infectious pancreatic necrosis virus from hatchery water using positively charged 1-MDS filters. The method consists of passing large volumes (Ca. 1001) of hatchery water through 1-MDS microporous filter followed by the elution of the adsorbed virus using a high pH buffer. The virus adsorbed efficiently to 1-MDS filters when the pH of the water was 5.5 and was eluted optimally with 3% beef extract solution (pH 10). This procedure permitted the processing of 100 1 of hatchery water which resulted in a 300-fold reduction in the volume of water and greater than 90% recovery of the seeded virus.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalJournal of Virological Methods
Volume31
Issue number2-3
DOIs
StatePublished - Jan 1 1991

Keywords

  • Aquaculture
  • Fish virus
  • Infectious pancreatic necrosis virus
  • Viradel procedure
  • Virus concentration

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