Method for the concentration of infectious pancreatic necrosis virus from hatchery water

S. Maheshkumar; Sagar M. Goyal; Richard B. Peterson; Philip P. Economon

doi:10.1016/0166-0934(91)90159-W

Method for the concentration of infectious pancreatic necrosis virus from hatchery water

S. Maheshkumar, Sagar M. Goyal, Richard B. Peterson, Philip P. Economon

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A method was developed for concentrating infectious pancreatic necrosis virus from hatchery water using positively charged 1-MDS filters. The method consists of passing large volumes (Ca. 1001) of hatchery water through 1-MDS microporous filter followed by the elution of the adsorbed virus using a high pH buffer. The virus adsorbed efficiently to 1-MDS filters when the pH of the water was 5.5 and was eluted optimally with 3% beef extract solution (pH 10). This procedure permitted the processing of 100 1 of hatchery water which resulted in a 300-fold reduction in the volume of water and greater than 90% recovery of the seeded virus.

Original language	English (US)
Pages (from-to)	211-217
Number of pages	7
Journal	Journal of Virological Methods
Volume	31
Issue number	2-3
DOIs	https://doi.org/10.1016/0166-0934(91)90159-W
State	Published - 1991

Keywords

Aquaculture
Fish virus
Infectious pancreatic necrosis virus
Viradel procedure
Virus concentration

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0166-0934(91)90159-W

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title = "Method for the concentration of infectious pancreatic necrosis virus from hatchery water",

abstract = "A method was developed for concentrating infectious pancreatic necrosis virus from hatchery water using positively charged 1-MDS filters. The method consists of passing large volumes (Ca. 1001) of hatchery water through 1-MDS microporous filter followed by the elution of the adsorbed virus using a high pH buffer. The virus adsorbed efficiently to 1-MDS filters when the pH of the water was 5.5 and was eluted optimally with 3% beef extract solution (pH 10). This procedure permitted the processing of 100 1 of hatchery water which resulted in a 300-fold reduction in the volume of water and greater than 90% recovery of the seeded virus.",

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year = "1991",

doi = "10.1016/0166-0934(91)90159-W",

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T1 - Method for the concentration of infectious pancreatic necrosis virus from hatchery water

AU - Maheshkumar, S.

AU - Goyal, Sagar M.

AU - Peterson, Richard B.

AU - Economon, Philip P.

PY - 1991

Y1 - 1991

N2 - A method was developed for concentrating infectious pancreatic necrosis virus from hatchery water using positively charged 1-MDS filters. The method consists of passing large volumes (Ca. 1001) of hatchery water through 1-MDS microporous filter followed by the elution of the adsorbed virus using a high pH buffer. The virus adsorbed efficiently to 1-MDS filters when the pH of the water was 5.5 and was eluted optimally with 3% beef extract solution (pH 10). This procedure permitted the processing of 100 1 of hatchery water which resulted in a 300-fold reduction in the volume of water and greater than 90% recovery of the seeded virus.

AB - A method was developed for concentrating infectious pancreatic necrosis virus from hatchery water using positively charged 1-MDS filters. The method consists of passing large volumes (Ca. 1001) of hatchery water through 1-MDS microporous filter followed by the elution of the adsorbed virus using a high pH buffer. The virus adsorbed efficiently to 1-MDS filters when the pH of the water was 5.5 and was eluted optimally with 3% beef extract solution (pH 10). This procedure permitted the processing of 100 1 of hatchery water which resulted in a 300-fold reduction in the volume of water and greater than 90% recovery of the seeded virus.

KW - Aquaculture

KW - Fish virus

KW - Infectious pancreatic necrosis virus

KW - Viradel procedure

KW - Virus concentration

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

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ER -

Method for the concentration of infectious pancreatic necrosis virus from hatchery water

Abstract

Keywords

UN SDGs

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