Method for the assay of free and esterified tocopherols

C. K. Chow; H. H. Draper; A. Saari Csallany

doi:10.1016/0003-2697(69)90106-7

Method for the assay of free and esterified tocopherols

C. K. Chow, H. H. Draper, A. Saari Csallany

Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

A method for estimating the eight naturally occurring isomers of vitamin E and their esters is described. The prime feature of this method is the removal of the bulk of the lipids by low-temperature crystallization instead of saponification. The technique concomitantly affords a separation of tocopherol esters from free tocopherols. The recovery of tocopherols was superior to that obtained by the conventional saponification method, which was shown to cause extensive destruction of tocotrienols. Reduction with LiAlH₄ provided the highest yield of free tocopherols from tocopherol esters. The conjugated forms were found only in latex lipids. Analytical values are given for the tocopherols in several plant oils.

Original language	English (US)
Pages (from-to)	81-90
Number of pages	10
Journal	Analytical Biochemistry
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(69)90106-7
State	Published - Oct 1969

Publisher link

10.1016/0003-2697(69)90106-7

Find It

Find It at U of M Libraries

Cite this

@article{d82f34c33c8245b6b7b0420f998259a2,

title = "Method for the assay of free and esterified tocopherols",

abstract = "A method for estimating the eight naturally occurring isomers of vitamin E and their esters is described. The prime feature of this method is the removal of the bulk of the lipids by low-temperature crystallization instead of saponification. The technique concomitantly affords a separation of tocopherol esters from free tocopherols. The recovery of tocopherols was superior to that obtained by the conventional saponification method, which was shown to cause extensive destruction of tocotrienols. Reduction with LiAlH4 provided the highest yield of free tocopherols from tocopherol esters. The conjugated forms were found only in latex lipids. Analytical values are given for the tocopherols in several plant oils.",

author = "Chow, {C. K.} and Draper, {H. H.} and {Saari Csallany}, A.",

year = "1969",

month = oct,

doi = "10.1016/0003-2697(69)90106-7",

language = "English (US)",

volume = "32",

pages = "81--90",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Method for the assay of free and esterified tocopherols

AU - Chow, C. K.

AU - Draper, H. H.

AU - Saari Csallany, A.

PY - 1969/10

Y1 - 1969/10

N2 - A method for estimating the eight naturally occurring isomers of vitamin E and their esters is described. The prime feature of this method is the removal of the bulk of the lipids by low-temperature crystallization instead of saponification. The technique concomitantly affords a separation of tocopherol esters from free tocopherols. The recovery of tocopherols was superior to that obtained by the conventional saponification method, which was shown to cause extensive destruction of tocotrienols. Reduction with LiAlH4 provided the highest yield of free tocopherols from tocopherol esters. The conjugated forms were found only in latex lipids. Analytical values are given for the tocopherols in several plant oils.

AB - A method for estimating the eight naturally occurring isomers of vitamin E and their esters is described. The prime feature of this method is the removal of the bulk of the lipids by low-temperature crystallization instead of saponification. The technique concomitantly affords a separation of tocopherol esters from free tocopherols. The recovery of tocopherols was superior to that obtained by the conventional saponification method, which was shown to cause extensive destruction of tocotrienols. Reduction with LiAlH4 provided the highest yield of free tocopherols from tocopherol esters. The conjugated forms were found only in latex lipids. Analytical values are given for the tocopherols in several plant oils.

UR - http://www.scopus.com/inward/record.url?scp=0014679382&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014679382&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(69)90106-7

DO - 10.1016/0003-2697(69)90106-7

M3 - Article

C2 - 5407965

AN - SCOPUS:0014679382

SN - 0003-2697

VL - 32

SP - 81

EP - 90

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Method for the assay of free and esterified tocopherols

Abstract

Publisher link

Find It

Other files and links

Fingerprint

Cite this